BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5253

Title: Structural Differences in the NOE-derived Structure of G-T Mismatched DNA relative to Normal DNA are Correlated with Differences in (13)C Relaxation-based Internal Dynamics   PubMed: 12051946

Authors: Isaacs, R.; Rayens, W.; Spielmann, H.

Citation: Isaacs, R.; Rayens, W.; Spielmann, H.. "Structural Differences in the NOE-derived Structure of G-T Mismatched DNA relative to Normal DNA are Correlated with Differences in (13)C Relaxation-based Internal Dynamics"  J. Mol. Biol. 319, 191-207 (2002).

Assembly members:
5'-D(*CP*CP*AP*TP*GP*CP*GP*TP*GP*G)-3', polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
5'-D(*CP*CP*AP*TP*GP*CP*GP*TP*GP*G)-3': CCATGCGTGG

Data sets:
Data typeCount
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(*CP*CP*AP*TP*GP*CP*GP*TP*GP*G)-3'1
25'-D(*CP*CP*AP*TP*GP*CP*GP*TP*GP*G)-3'1

Entities:

Entity 1, 5'-D(*CP*CP*AP*TP*GP*CP*GP*TP*GP*G)-3' 10 residues - Formula weight is not available

1   DCDCDADTDGDCDGDTDGDG

Samples:

sample_1: 5'-D(*CP*CP*AP*TP*GP*CP*GP*TP*GP*G)-3' 8 mL; NaCl 200 mM; NaPO4 20 mM; NaN3 10 mM; EDTA 0.1 mM; D2O 99.96%

sample_cond_1: ionic strength: 0.230 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1

Software:

VNMR v6b - collection

FELIX v98.0 - data analysis

MARDIGRAS v1 - iterative matrix relaxation

DISCOVER v2.98 - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links: