BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5261

Title: Nuclease A Inhibitor (NuiA) Assignments   PubMed: 12095254

Authors: Kirby, Thomas; Mueller, Geoffrey; DeRose, Eugene; Lebetkin, Mark; Meiss, Gregor; Pingoud, Alfred; London, Robert

Citation: Kirby, Thomas; Mueller, Geoffrey; DeRose, Eugene; Lebetkin, Mark; Meiss, Gregor; Pingoud, Alfred; London, Robert. "The Nuclease A Inhibitor represents a new Variation of the Rare PR-1 Fold"  J. Mol. Biol. 320, 771-782 (2002).

Assembly members:
Nuclease A Inhibitor, polymer, 143 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena PCC 7120

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Nuclease A Inhibitor: MHHHHHHGSTKTNSEILEQL KQASDGLLFMSESEYPFEVF LWEGSAPPVTHEIVLQQTGH GQDAPFKVVDIDSFFSRATT PQDWYEDEENAVVAKFQKLL EVIKSNLKNPQVYRLGEVEL DVYVIGETPAGNLAGISTKV VET

Data sets:
Data typeCount
1H chemical shifts936
13C chemical shifts589
15N chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Nuclease A Inhibitor1

Entities:

Entity 1, Nuclease A Inhibitor 143 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYSERTHR
2   LYSTHRASNSERGLUILELEUGLUGLNLEU
3   LYSGLNALASERASPGLYLEULEUPHEMET
4   SERGLUSERGLUTYRPROPHEGLUVALPHE
5   LEUTRPGLUGLYSERALAPROPROVALTHR
6   HISGLUILEVALLEUGLNGLNTHRGLYHIS
7   GLYGLNASPALAPROPHELYSVALVALASP
8   ILEASPSERPHEPHESERARGALATHRTHR
9   PROGLNASPTRPTYRGLUASPGLUGLUASN
10   ALAVALVALALALYSPHEGLNLYSLEULEU
11   GLUVALILELYSSERASNLEULYSASNPRO
12   GLNVALTYRARGLEUGLYGLUVALGLULEU
13   ASPVALTYRVALILEGLYGLUTHRPROALA
14   GLYASNLEUALAGLYILESERTHRLYSVAL
15   VALGLUTHR

Samples:

sample_1: Nuclease A Inhibitor, [U-13C; U-15N], 3.3 mM; Tris, [U-2H], 90 mM

condition_1: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecondition_1

Software:

nmrDraw v1.8 -

NMRVIEW v5.0.4 - analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB77119
EMBL CAA54677
GB ABA25047
REF WP_010999910 WP_011316624 YP_227662 YP_320236