BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5262

Title: Backbone 1H and 15N Chemical Shift Assignements for the first fibronectin type II module of MMP-2 (col-1)   PubMed: 11928808

Authors: Gehrmann, Marion; Briknarova, Klara; Banyai, Laszlo; Patthy, Laszlo; Llinas, Miguel

Citation: Gehrmann, Marion; Briknarova, Klara; Banyai, Laszlo; Patthy, Laszlo; Llinas, Miguel. "The Col-1 Module of Human Matrix Metalloproteinase-2 (MMP-2): Structural/Functional Relatedness between Gelatin-binding Fibronectin Type II Modules and Lysine-binding Kringle Domains "  Biol. Chem. 383, 137-148 (2002).

Assembly members:
the first fibronectin type II module of human matrix metalloproteinase, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
the first fibronectin type II module of human matrix metalloproteinase: RIPVKYGNADGEYCKFPFLF NGKEYNSCTDTGRSDGFLWC STTYNFEKDGKYGFCPHEAL

Data sets:
Data typeCount
1H chemical shifts397
15N chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1col-11

Entities:

Entity 1, col-1 60 residues - Formula weight is not available

1   ARGILEPROVALLYSTYRGLYASNALAASP
2   GLYGLUTYRCYSLYSPHEPROPHELEUPHE
3   ASNGLYLYSGLUTYRASNSERCYSTHRASP
4   THRGLYARGSERASPGLYPHELEUTRPCYS
5   SERTHRTHRTYRASNPHEGLULYSASPGLY
6   LYSTYRGLYPHECYSPROHISGLUALALEU

Samples:

sample_1: the first fibronectin type II module of human matrix metalloproteinase 0.5 mM; H2O 90%; acetone, [U-2H], 10%

sample_2: the first fibronectin type II module of human matrix metalloproteinase 0.5 mM

sample_3: the first fibronectin type II module of human matrix metalloproteinase, [U-15N], 0.7 mM

condition_1: pH*: 7.3; temperature: 298 K

condition_2: pH*: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D 15N-edited TOCSYnot availablenot availablenot available
3D 15N-edited NOESYnot availablenot availablenot available

Software:

XWINNMR v2.0 - experiment acquisition

FELIX v98 - data processing and assignment

XPLOR v3.851 - structure calculation

NMR spectrometers:

  • Bruker AvanceDMX 500 MHz

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