BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5264

Title: Solution Structure of Human beta-Defensin 3   PubMed: 11741980

Authors: Schibli, D.; Hunter, H.; Aseyev, V.; Starner, T.; Wiencek, J.; McCray, P.; Tack, B.; Vogel, H.

Citation: Schibli, D.; Hunter, H.; Aseyev, V.; Starner, T.; Wiencek, J.; McCray, P.; Tack, B.; Vogel, H.. "The Solution Structures of the Human beta-Defensins lead to a Better Understanding of the Potent Bactericidal Activity of HBD3 against Staphylococcus aureus"  J. Biol. Chem. 277, 8279-8289 (2002).

Assembly members:
Human beta-defensin 3, polymer, 45 residues, 5168.9 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Human beta-defensin 3: GIINTLQKYYCRVRGGRCAV LSCLPKEEQIGKCSTRGRKC CRRKK

Data sets:
Data typeCount
1H chemical shifts279

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Beta-defensin 31

Entities:

Entity 1, Beta-defensin 3 45 residues - 5168.9 Da.

1   GLYILEILEASNTHRLEUGLNLYSTYRTYR
2   CYSARGVALARGGLYGLYARGCYSALAVAL
3   LEUSERCYSLEUPROLYSGLUGLUGLNILE
4   GLYLYSCYSSERTHRARGGLYARGLYSCYS
5   CYSARGARGLYSLYS

Samples:

sample_1: Human beta-defensin 3 0.36 mM; D2O 10%; H2O 90%

sample_2: Human beta-defensin 3 0.36 mM; D2O 100%

sample_cond_1: pH: 4.00; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D DQF-COSYnot availablenot availablenot available

Software:

XWINNMR v2.6 - collection

NMRPipe v1.8 - processing

NMRView v4.1.3 - data analysis

CNS v1.0 - refinement

Aria v1.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB40572 BAJ20226
EMBL CAC03097 CAL68919 CAL68920
GB AAF73853 AAG02237 AAG22030 AAI11597 AAI48675
REF NP_001075020 NP_001123222 NP_061131 XP_001083420 XP_002756804
SP A4H1Z9 A4H200 P81534 Q95JD2
TPG DAA01350