BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5278

Title: PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure   PubMed: 12225754

Authors: Nixon, P.; Giedroc, D.

Citation: Nixon, P.; Rangan, A.; Kim, Y-G.; Rich, A.; Hoffman, D.; Hennig, M.; Giedroc, D.. "Solution Structure of a Luteoviral P1-P2 frameshifting mRNA Pseudoknot"  J. Mol. Biol. 322, 621-633 (2002).

Assembly members:
Pea Enation Mosaic Virus Pseudoknot, polymer, 33 residues, Formula weight is not available

Natural source:   Common Name: Pea enation mosaic virus   Taxonomy ID: 12290   Superkingdom: Viruses   Kingdom: not available   Genus/species: Enamovirus Pea enation mosaic virus

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
Pea Enation Mosaic Virus Pseudoknot: GAAUUCCGGUXGACUCCGGA GAAACAAAGUCAA

Data sets:
Data typeCount
1H chemical shifts173
13C chemical shifts36
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P1-P2 frameshifting pseudoknot1

Entities:

Entity 1, P1-P2 frameshifting pseudoknot 33 residues - Formula weight is not available

1   GAAUUCCGGU
2   CHGACUCCGGA
3   GAAACAAAGU
4   CAA

Samples:

sample_1: Pea Enation Mosaic Virus Pseudoknot 2 mM; KPO4 10 mM; KCl 100 mM; MgCl2 5 mM

sample_2: Pea Enation Mosaic Virus Pseudoknot, [U-95% 15N], 0.2 mM; KPO4 10 mM; KCl 100 mM; MgCl2 5 mM

sample_3: Pea Enation Mosaic Virus Pseudoknot, [U-95% 13C; U-95% 15N], 0.3 mM; KPO4 10 mM; KCl 100 mM; MgCl2 5 mM

sample_cond_1: pH: 6.0; temperature: 283 K; ionic strength: 110 mM; pressure: 1 atm

sample_cond_2: pH: 6.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_3: pH: 5.8; temperature: 283 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available

Software:

X-PLOR v3.851 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links: