BMRB Entry 5279

Title:
A Quick Solution Structure Determination of the Fully Oxidized Double Mutant K9-10A Cytochrome c7 from Desulfuromonas acetoxidans and Mechanistic Implications
Deposition date:
2002-02-06
Original release date:
2002-04-01
Authors:
Assfalg, M.; Bertini, I.; Turano, P.; Bruschi, M.; Durand, M.; Giudici-Orticoni, M.; Dolla, A.
Citation:

Citation: Assfalg, M.; Bertini, I.; Turano, P.; Bruschi, M.; Durand, M.; Giudici-Orticoni, M.; Dolla, A.. "A Quick Solution Structure Determination of the Fully Oxidized Double Mutant K9-10A Cytochrome c7 from Desulfuromonas acetoxidans and Mechanistic Implications "  J. Biomol. NMR 22, 107-122 (2002).

Assembly members:

Assembly members:
cytochrome c7, polymer, 68 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 891   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfuromonas acetoxidans

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data typeCount
1H chemical shifts450

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c71
2heme c, 12
3heme c, 22
4heme c, 32

Entities:

Entity 1, cytochrome c7 68 residues - Formula weight is not available

1   ALAASPVALVALTHRTYRGLUASNALAALA
2   GLYASNVALTHRPHEASPHISLYSALAHIS
3   ALAGLULYSLEUGLYCYSASPALACYSHIS
4   GLUGLYTHRPROALALYSILEALAILEASP
5   LYSLYSSERALAHISLYSASPALACYSLYS
6   THRCYSHISLYSSERASNASNGLYPROTHR
7   LYSCYSGLYGLYCYSHISILELYS

Entity 2, heme c, 1 - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c7 2 mM; oxidized heme c 6 mM; sodium phosphate buffer 100 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1

Software:

PSEUDYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB