BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5292

Title: 1H Chemical Shift Assignments for TC5b   PubMed: 11979279

Authors: Neidigh, Jonathan; Fesinmeyer, R.; Andersen, Niels

Citation: Neidigh, Jonathan; Fesinmeyer, R.; Andersen, Niels. "Designing a 20-residue Protein"  Nat. Struct. Biol. 9, 425-430 (2002).

Assembly members:
Trp-cage Construct 5b, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Trp-cage Construct 5b: NLYIQWLKDGGPSSGRPPPS

Data sets:
Data typeCount
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TC5b1

Entities:

Entity 1, TC5b 20 residues - Formula weight is not available

1   ASNLEUTYRILEGLNTRPLEULYSASPGLY
2   GLYPROSERSERGLYARGPROPROPROSER

Samples:

sample_1: Trp-cage Construct 5b1.0 – 1.8 mM; phosphate buffer 15 mM; H2O 100%

sample_2: Trp-cage Construct 5b1.0 – 1.8 mM; phosphate buffer 15 mM; Trifluoroethanol 30%; H2O 70%

Aqueous_Buffer: pH: 7; temperature: 282 K

TFE: pH: 7; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
TOCSY (15-60ms spinlock)not availablenot availablenot available
NOESY (60-200ms mixing time)not availablenot availablenot available

Software:

xwinnmr - data collection

FELIX v95 - manual peak assignment, processing

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

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