BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5299

Title: The Mad2 Spindle Checkpoint Protein Undergoes Similar Major Conformational Changes upon Binding to Either Mad1 or Cdc20   PubMed: 11804586

Authors: Luo, X.; Tang, Z.; Rizo, J.; Yu, H.

Citation: Luo, X.; Tang, Z.; Rizo, J.; Yu, H.. "The Mad2 Spindle Checkpoint Protein Undergoes Similar Major Conformational Changes upon Binding to Either Mad1 or Cdc20 "  Mol. Cell 9, 59-71 (2002).

Assembly members:
mitotic checkpoint protein Mad2, polymer, 197 residues, Formula weight is not available
Mad2-binding peptide, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
mitotic checkpoint protein Mad2: GSITLRGSAEIVAEFFSFGI NSILYQRGIYPSETFTRVQK YGLTLLVTTDLELIKYLNNV VEQLKDWLYKCSVQKLVVVI SNIESGEVLERWQFDIECDK TAKDDSAPREKSQKAIQDEI RSVIRQITATVTFLPLLEVS CSFDLLIYTDKDLVVPEKWE ESGPQFITNSEEVRLRSFTT TIHKVNSMVAYKIPVND
Mad2-binding peptide: SWYSYPPPQRAV

Data sets:
Data typeCount
1H chemical shifts1183
13C chemical shifts749
15N chemical shifts213

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A1
2Mad2-binding peptide2

Entities:

Entity 1, MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A 197 residues - Formula weight is not available

1   GLYSERILETHRLEUARGGLYSERALAGLU
2   ILEVALALAGLUPHEPHESERPHEGLYILE
3   ASNSERILELEUTYRGLNARGGLYILETYR
4   PROSERGLUTHRPHETHRARGVALGLNLYS
5   TYRGLYLEUTHRLEULEUVALTHRTHRASP
6   LEUGLULEUILELYSTYRLEUASNASNVAL
7   VALGLUGLNLEULYSASPTRPLEUTYRLYS
8   CYSSERVALGLNLYSLEUVALVALVALILE
9   SERASNILEGLUSERGLYGLUVALLEUGLU
10   ARGTRPGLNPHEASPILEGLUCYSASPLYS
11   THRALALYSASPASPSERALAPROARGGLU
12   LYSSERGLNLYSALAILEGLNASPGLUILE
13   ARGSERVALILEARGGLNILETHRALATHR
14   VALTHRPHELEUPROLEULEUGLUVALSER
15   CYSSERPHEASPLEULEUILETYRTHRASP
16   LYSASPLEUVALVALPROGLULYSTRPGLU
17   GLUSERGLYPROGLNPHEILETHRASNSER
18   GLUGLUVALARGLEUARGSERPHETHRTHR
19   THRILEHISLYSVALASNSERMETVALALA
20   TYRLYSILEPROVALASNASP

Entity 2, Mad2-binding peptide 12 residues - Formula weight is not available

1   SERTRPTYRSERTYRPROPROPROGLNARG
2   ALAVAL

Samples:

sample_1: mitotic checkpoint protein Mad2, [U-15N], 0.8 mM; Mad2-binding peptide 1 mM; phosphate buffer 50 mM; KCl 0.3 M; DTT 1 mM; D2O 10%; H2O 90%

sample_2: mitotic checkpoint protein Mad2, [U-15N; U-13C; U-2H], 0.8 mM; Mad2-binding peptide 1 mM; phosphate buffer 50 mM; KCl 0.3 M; DTT 1 mM; D2O 10%; H2O 90%

sample_3: mitotic checkpoint protein Mad2, [U-15N; U-13C], 0.8 mM; Mad2-binding peptide 1 mM; phosphate buffer 50 mM; KCl 0.3 M; DTT 1 mM; D2O 10%; H2O 90%

sample_4: mitotic checkpoint protein Mad2, [U-15N; U-13C; U-60% 2H], 0.8 mM; Mad2-binding peptide 1 mM; phosphate buffer 50 mM; KCl 0.3 M; DTT 1 mM; D2O 10%; H2O 90%

sample_5: mitotic checkpoint protein Mad2 1 mM; Mad2-binding peptide, [U-13C; U-15N], 0.8 mM; phosphate buffer 50 mM; KCl 0.3 M; DTT 1 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.4; temperature: 303 K; ionic strength: 0.3 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
3D H(CC)(CO)NHnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
3D (H)C(C)(CO)NHnot availablenot availablenot available
HN(COCA)CBnot availablenot availablenot available
HN(CA)CBnot availablenot availablenot available

Software:

CNS v0.9a - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

REF XP_001084989 NP_002349
SWISS-PROT Q13257
GenBank AAH70283 AAH00356 AAH05945 AAC50781 AAC52060
DBJ BAG36562 BAD97153 BAE01009 BAB63410
EMBL CAA03943
PDB
BMRB 4775 5299