BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5303

Title: Solution structure and backbone dynamics of beryllofluoride-activated NTRC receiver domain   PubMed: 12885241

Authors: Hastings, C.; Lee, S.-Y.; Cho, H.; Yan, D.; Kustu, S.; Wemmer, D.

Citation: Hastings, C.; Lee, S-Y.; Cho, H.; Yan, D.; Kustu, S.; Wemmer, D.. "High-resolution Solution Structure of the Beryllofluoride-activated NtrC Receiver Domain "  Biochemistry 42, 9081-9090 (2003).

Assembly members:
nitrogen regulation protein NR(I), polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 602   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
nitrogen regulation protein NR(I): MQRGIVWVVDDDSSIRWVLE RALAGAGLTCTTFENGNEVL AALASKTPDVLLSDIRMPGM DGLALLKQIKQRHPMLPVII MTAHSDLDAAVSAYQQGAFD YLPKPFDIDEAVALVERAIS HYQE

Data sets:
Data typeCount
1H chemical shifts804
13C chemical shifts371
15N chemical shifts117
coupling constants76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nitrogen regulation protein NR(I)1

Entities:

Entity 1, nitrogen regulation protein NR(I) 124 residues - Formula weight is not available

1   METGLNARGGLYILEVALTRPVALVALASP
2   ASPASPSERSERILEARGTRPVALLEUGLU
3   ARGALALEUALAGLYALAGLYLEUTHRCYS
4   THRTHRPHEGLUASNGLYASNGLUVALLEU
5   ALAALALEUALASERLYSTHRPROASPVAL
6   LEULEUSERASPILEARGMETPROGLYMET
7   ASPGLYLEUALALEULEULYSGLNILELYS
8   GLNARGHISPROMETLEUPROVALILEILE
9   METTHRALAHISSERASPLEUASPALAALA
10   VALSERALATYRGLNGLNGLYALAPHEASP
11   TYRLEUPROLYSPROPHEASPILEASPGLU
12   ALAVALALALEUVALGLUARGALAILESER
13   HISTYRGLNGLU

Samples:

sample_1: nitrogen regulation protein NR(I), [U-15N], 1.1 – 1.5 mM; sodium phosphate buffer 50 mM; NaCl 50 mM; BeCl2 4.4 mM; MgCl2 7.2 mM; NaF 29 mM; H2O 95%; D2O 5%

sample_2: nitrogen regulation protein NR(I), [U-13C; U-15N], 1.1 – 1.5 mM; sodium phosphate buffer 50 mM; NaCl 50 mM; BeCl2 4.4 mM; MgCl2 7.2 mM; NaF 29 mM; H2O 95%; D2O 5%

sample_3: nitrogen regulation protein NR(I), [U-10% 13C], 1.1 – 1.5 mM; sodium phosphate buffer 50 mM; NaCl 50 mM; BeCl2 4.4 mM; MgCl2 7.2 mM; NaF 29 mM; H2O 95%; D2O 5%

sample_4: nitrogen regulation protein NR(I), [U-15N], 1.1 – 1.5 mM; sodium phosphate buffer 50 mM; NaCl 50 mM; BeCl2 4.4 mM; MgCl2 7.2 mM; NaF 29 mM; H2O 95%; D2O 5%; phage pf1 30 mg/mL

sample_cond_1: pH: 6.75; temperature: 303 K; ionic strength: 140 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
4D 13C/15N-separated NOESYnot availablenot availablenot available
4D 13C-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNCA-Jnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
13C,1H-HSQCnot availablenot availablenot available
IPAP-HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

DYANA v1.5 - structure solution

X-PLOR vCNS 1.0 - structure solution

FELIX - processing

NMRVIEW - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 4527 4528 4762 4763 4764
PDB
DBJ BAB38213 BAE77441 BAG79675 BAI27885 BAI33008
EMBL CAA28808 CAA59425 CAD03095 CAP78325 CAQ34219
GB AAB03002 AAC76865 AAG59057 AAL22844 AAN45373
PIR AC0950 E86074
REF NP_290493 NP_312817 NP_418304 NP_458044 NP_462885
SP P0AFB8 P0AFB9 P41789