BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5312

Title: 1H, 13C and 15N chemical shift assignments of aIF2beta from Methanobacterium themoautotrophicum   PubMed: 12062427

Authors: Gutierrez, Pablo; Coillet-Matillon, Stephane; Arrowsmith, Cheryl; Gehring, Kalle

Citation: Gutierrez, Pablo; Coillet-Matillon, Stephane; Arrowsmith, Cheryl; Gehring, Kalle. "Zinc is required for Structural Stability of the C-terminus of Archaeal Translation Initiation Factor aIF2beta "  FEBS Lett. 517, 155-158 (2002).

Assembly members:
translation initiation factor 2 beta, polymer, 135 residues, 16200 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
translation initiation factor 2 beta: MDDYEKLLERAIDQLPPEVF ETKRFEVPKAYSVIQGNRTF IQNFREVADALNRDPQHLLK FLLRELGTAGNLEGGRAILQ GKFTHFLINERIEDYVNKFV ICHECNRPDTRIIREGRISL LKCEACGAKAPLKNV

Data sets:
Data typeCount
1H chemical shifts518
13C chemical shifts296
15N chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aIF2beta1
2Zinc ion2

Entities:

Entity 1, aIF2beta 135 residues - 16200 Da.

1   METASPASPTYRGLULYSLEULEUGLUARG
2   ALAILEASPGLNLEUPROPROGLUVALPHE
3   GLUTHRLYSARGPHEGLUVALPROLYSALA
4   TYRSERVALILEGLNGLYASNARGTHRPHE
5   ILEGLNASNPHEARGGLUVALALAASPALA
6   LEUASNARGASPPROGLNHISLEULEULYS
7   PHELEULEUARGGLULEUGLYTHRALAGLY
8   ASNLEUGLUGLYGLYARGALAILELEUGLN
9   GLYLYSPHETHRHISPHELEUILEASNGLU
10   ARGILEGLUASPTYRVALASNLYSPHEVAL
11   ILECYSHISGLUCYSASNARGPROASPTHR
12   ARGILEILEARGGLUGLYARGILESERLEU
13   LEULYSCYSGLUALACYSGLYALALYSALA
14   PROLEULYSASNVAL

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: translation initiation factor 2 beta, [U-13C; U-15N], 1.0 – 2.0 mM; ZINC (II) ION 51 mM

sample_2: translation initiation factor 2 beta, [U-15N], 1.0 – 2.0 mM; ZINC (II) ION 51 mM

condition_1: pH: 6.0 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablecondition_1
1H-15N TOCSYnot availablenot availablecondition_1
HNCAnot availablenot availablecondition_1
HNCACBnot availablenot availablecondition_1
CBCA(CO)NHnot availablenot availablecondition_1
1H-13C NOESYnot availablenot availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian UNITYplus 800 MHz

Related Database Links:

REF NP_276875
SWISS-PROT O27797
PDB
GenBank AAB86235