BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5316

Title: Structure of the N-terminal 283-Residue Fragment of the HIV-1 Gag Polyprotein   PubMed: 12032547

Authors: Tang, Chun; Ndassa, Yasmine; Summers, Michael

Citation: Tang, Chun; Ndassa, Yasmine; Summers, Michael. "Structure of the N-terminal 283-Residue Fragment of the HIV-1 Gag Polyprotein"  Nat. Struct. Biol. 9, 537-543 (2002).

Assembly members:
matrix-capsid, polymer, 288 residues, 32216.6 Da.

Natural source:   Common Name: HIV   Taxonomy ID: 12721   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus Human immunodeficiency virus type 1

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
matrix-capsid: GARASVLSGGELDKWEKIRL RPGGKKQYKLKHIVWASREL ERFAVNPGLLETSEGCRQIL GQLQPSLQTGSEELRSLYNT IAVLYCVHQRIDVKDTKEAL DKIEEEQNKSKKKAQQAAAD TGNNSQVSQNYPIVQNLQGQ MVHQAISPRTLNAWVKVVEE KAFSPEVIPMFSALSEGATP QDLNTMLNTVGGHQAAMQML KETINEEAAEWDRLHPVHAG PIAPGQMREPRGSDIAGTTS TLQEQIGWMTHNPPIPVGEI YKRWIILGLNKIVRMYSPTS ILHHHHHH

Data sets:
Data typeCount
1H chemical shifts1636
13C chemical shifts1138
15N chemical shifts273

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Gag2831

Entities:

Entity 1, Gag283 288 residues - 32216.6 Da.

1   GLYALAARGALASERVALLEUSERGLYGLY
2   GLULEUASPLYSTRPGLULYSILEARGLEU
3   ARGPROGLYGLYLYSLYSGLNTYRLYSLEU
4   LYSHISILEVALTRPALASERARGGLULEU
5   GLUARGPHEALAVALASNPROGLYLEULEU
6   GLUTHRSERGLUGLYCYSARGGLNILELEU
7   GLYGLNLEUGLNPROSERLEUGLNTHRGLY
8   SERGLUGLULEUARGSERLEUTYRASNTHR
9   ILEALAVALLEUTYRCYSVALHISGLNARG
10   ILEASPVALLYSASPTHRLYSGLUALALEU
11   ASPLYSILEGLUGLUGLUGLNASNLYSSER
12   LYSLYSLYSALAGLNGLNALAALAALAASP
13   THRGLYASNASNSERGLNVALSERGLNASN
14   TYRPROILEVALGLNASNLEUGLNGLYGLN
15   METVALHISGLNALAILESERPROARGTHR
16   LEUASNALATRPVALLYSVALVALGLUGLU
17   LYSALAPHESERPROGLUVALILEPROMET
18   PHESERALALEUSERGLUGLYALATHRPRO
19   GLNASPLEUASNTHRMETLEUASNTHRVAL
20   GLYGLYHISGLNALAALAMETGLNMETLEU
21   LYSGLUTHRILEASNGLUGLUALAALAGLU
22   TRPASPARGLEUHISPROVALHISALAGLY
23   PROILEALAPROGLYGLNMETARGGLUPRO
24   ARGGLYSERASPILEALAGLYTHRTHRSER
25   THRLEUGLNGLUGLNILEGLYTRPMETTHR
26   HISASNPROPROILEPROVALGLYGLUILE
27   TYRLYSARGTRPILEILELEUGLYLEUASN
28   LYSILEVALARGMETTYRSERPROTHRSER
29   ILELEUHISHISHISHISHISHIS

Samples:

sample_1: matrix-capsid, [U-100% 13C; U-100% 15N], 1.0 mM; sodium acetate 50 mM; NaCl 100 mM; beta-mecaptoethanol 5 mM

cond_1: pH: 5.0; temperature: 303.3 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-15N TROSYnot availablenot availablenot available
15N-edited NOESY-HSQCnot availablenot availablenot available
15N-edited TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNHAnot availablenot availablenot available
15N,15N-edited HSQC-NOESY-HSQCnot availablenot availablenot available
15N,13C-edited HSQC-NOESY-HMQCnot availablenot availablenot available
13C,13C-edited HMQC-NOESY-HMQCnot availablenot availablenot available

Software:

NMRPIPE - spectra processing

NMRDRAW - spectra processing

NMRVIEW v5.0.3 - spectra analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 17738 19261 19264 19575
PDB
DBJ BAA00992 BAA93773 BAA93775 BAA93776 BAA93778
EMBL CAA65355 CAA76038 CAB85858 CAB85867 CAB85875
GB AAA44987 AAB04036 AAB60571 AAC28445 AAC54642
REF NP_579880
SP P12493 P12497