BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5317

Title: Solution structure of the DNA binding domain of human TRF1   PubMed: 11738049

Authors: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.

Citation: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.. "Solution structure of a telomeric DNA complex of human TRF1"  Structure 9, 1237-1251 (2001).

Assembly members:
TRF1, polymer, 69 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TRF1: TPEKHRARKRQAWLWEEDKN LRSGVRKYGEGNWSKILLHY KFNNRTSVMLKDRWRTMKKL KLISSDSED

Data sets:
Data typeCount
1H chemical shifts461
13C chemical shifts196
15N chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRF11

Entities:

Entity 1, TRF1 69 residues - Formula weight is not available

1   THRPROGLULYSHISARGALAARGLYSARG
2   GLNALATRPLEUTRPGLUGLUASPLYSASN
3   LEUARGSERGLYVALARGLYSTYRGLYGLU
4   GLYASNTRPSERLYSILELEULEUHISTYR
5   LYSPHEASNASNARGTHRSERVALMETLEU
6   LYSASPARGTRPARGTHRMETLYSLYSLEU
7   LYSLEUILESERSERASPSERGLUASP

Samples:

sample_1: TRF1, [U-15N; U-13C], 1.5 – 2.5 mM; phosphate buffer 50 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_2: TRF1, [U-15N; U-13C], 1.5 – 2.5 mM; phosphate buffer 50 mM; NaCl 100 mM; D2O 100%

sample_cond_1: pH: 6.8; temperature: 300 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D Sequential assignment protocolnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D DQF-COSYnot availablenot availablenot available

Software:

EMBOSS v5.0 - refinement

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4210 5361
PDB
DBJ BAE88046 BAF85218
EMBL CAA63768 CAH92488
GB AAB53363 AAB54036 AAB81137 AAC02531 AAH29378
REF NP_001126467 NP_003209 NP_059523 XP_001083645 XP_001164723
SP O55036 P54274