BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5318

Title: Backbone and Sidechain 1H, 13C and 15N resonance assignments for PLC-gamma 1 C-terminal SH2 domain   PubMed: 8181064

Authors: Forman-Kay, Julie

Citation: Pascal, Steve; Gish, Gerry; Yamazaki, Toshio; Singer, Alex; Shoelson, Steven; Pawson, Tony; Kay, Lewis; Forman-Kay, Julie. "Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-gamma 1 complexed with a high affinity binding peptide"  Cell 77, 461-472 (1994).

Assembly members:
PLCC SH2, polymer, 105 residues, Formula weight is not available

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PLCC SH2: GSPGIHESKEWYHASLTRAQ AEHMLMRVPRDGAFLVRKRN EPNSYAISFRAEGKIKHCRV QQEGQTVMLGNSEFDSLVDL ISYYEKHPLYRKMKLRYPIN EENSS

Data sets:
Data typeCount
15N chemical shifts98
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PLCC SH2 domain1

Entities:

Entity 1, PLCC SH2 domain 105 residues - Formula weight is not available

1   GLYSERPROGLYILEHISGLUSERLYSGLU
2   TRPTYRHISALASERLEUTHRARGALAGLN
3   ALAGLUHISMETLEUMETARGVALPROARG
4   ASPGLYALAPHELEUVALARGLYSARGASN
5   GLUPROASNSERTYRALAILESERPHEARG
6   ALAGLUGLYLYSILELYSHISCYSARGVAL
7   GLNGLNGLUGLYGLNTHRVALMETLEUGLY
8   ASNSERGLUPHEASPSERLEUVALASPLEU
9   ILESERTYRTYRGLULYSHISPROLEUTYR
10   ARGLYSMETLYSLEUARGTYRPROILEASN
11   GLUGLUASNSERSER

Samples:

sample_1: PLCC SH2, [U-99% 15N], 1.5 mM; sodium phosphate 100 mM

condition_1: pH: 6.4; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 13C- or 15N-edited NOESY-HSQCsample_1not availablecondition_1
3D 15N-edited TOCSY-HSQCsample_1not availablecondition_1
CN NOESY-HSQCsample_1not availablecondition_1
HBCBCA(CO)NNHsample_1not availablecondition_1
HNCACBsample_1not availablecondition_1
(HB)CB(CGCD)HDsample_1not availablecondition_1
(HB)CB(CGCDCE)HEsample_1not availablecondition_1
HMQC Jsample_1not availablecondition_1

Software:

X-PLOR - combined distance geometry-simulated annealing calculations

STEREOSEARCH - extraction of torsion angle restraint values

NMR spectrometers:

  • Varian UNITY 500 MHz

Related Database Links:

PDB