BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5320

Title: 1H and 15N Chemical Shift Assignments for Human B-FABP

Authors: Rademacher, Martin; Zimmerman, Aukje; Rueterjans, Heinz; Veerkamp, Jacques; Luecke, Christian

Citation: Rademacher, Martin; Zimmerman, Aukje; Rueterjans, Heinz; Veerkamp, Jacques; Luecke, Christian. "Solution structure of fatty acid-binding protein from human brain"  Mol. Cell. Biochem. 239, 61-68 (2002).

Assembly members:
brain-type fatty acid binding protein, polymer, 131 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
brain-type fatty acid binding protein: VEAFCATWKLTNSQNFDEYM KALGVGFATRQVGNVTKPTV IISQEGDKVVIRTLSTFKNT EISFQLGEEFDETTADDRNC KSVVSLDGDKLVHIQKWDGK ETNFVREIKDGKMVMTLTFG DVVAVRHYEKA

Data sets:
Data typeCount
1H chemical shifts924
15N chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B-FABP1

Entities:

Entity 1, B-FABP 131 residues - Formula weight is not available

1   VALGLUALAPHECYSALATHRTRPLYSLEU
2   THRASNSERGLNASNPHEASPGLUTYRMET
3   LYSALALEUGLYVALGLYPHEALATHRARG
4   GLNVALGLYASNVALTHRLYSPROTHRVAL
5   ILEILESERGLNGLUGLYASPLYSVALVAL
6   ILEARGTHRLEUSERTHRPHELYSASNTHR
7   GLUILESERPHEGLNLEUGLYGLUGLUPHE
8   ASPGLUTHRTHRALAASPASPARGASNCYS
9   LYSSERVALVALSERLEUASPGLYASPLYS
10   LEUVALHISILEGLNLYSTRPASPGLYLYS
11   GLUTHRASNPHEVALARGGLUILELYSASP
12   GLYLYSMETVALMETTHRLEUTHRPHEGLY
13   ASPVALVALALAVALARGHISTYRGLULYS
14   ALA

Samples:

sample_1: brain-type fatty acid binding protein2 – 4 mM

sample_2: brain-type fatty acid binding protein, [U-15N], 2 – 4 mM

cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N HTQCnot availablenot availablenot available
3D 1H-15N TOCSY-HSQCnot availablenot availablenot available
3D 1H-15N NOESY-HSQCnot availablenot availablenot available

Software:

XWINNMR v1.3 - processing

AURELIA v2.5.9 - analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 16042 16046 16047 16048 16049
PDB
DBJ BAA23324 BAA23645 BAD92052 BAE90445 BAF82525
EMBL CAA05773 CAC21646 CAG33338
GB AAB87141 AAD00507 AAH12299 AAY17257 ABM82363
REF NP_001020400 NP_001244643 NP_001270567 NP_001437 XP_002746967
SP O15540