BMRB Entry 5326
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5326
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Dmitriev, Oleg; Abildgaard, Frits; Markley, John; Fillingame, Robert. "Structure of Ala24/Asp61 --> Asp24/Asn61 Substituted Subunit C of Escherichia
coli ATP Synthase: Implications for the Mechanism of Proton Transport and
Rotary Movement in the F0 Complex" Biochemistry 41, 5537-5547 (2002).
PubMed: 11969414
Assembly members:
subunit c, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
subunit c: MENLNMDLLYMAAAVMMGLA
AIGDAIGIGILGGKFLEGAA
RQPDLIPLLRTQFFIVMGLV
NAIPMIAVGLGLYVMFAVA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 349 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 557 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit c | 1 |
Entities:
Entity 1, subunit c 79 residues - Formula weight is not available
| 1 | MET | GLU | ASN | LEU | ASN | MET | ASP | LEU | LEU | TYR | ||||
| 2 | MET | ALA | ALA | ALA | VAL | MET | MET | GLY | LEU | ALA | ||||
| 3 | ALA | ILE | GLY | ASP | ALA | ILE | GLY | ILE | GLY | ILE | ||||
| 4 | LEU | GLY | GLY | LYS | PHE | LEU | GLU | GLY | ALA | ALA | ||||
| 5 | ARG | GLN | PRO | ASP | LEU | ILE | PRO | LEU | LEU | ARG | ||||
| 6 | THR | GLN | PHE | PHE | ILE | VAL | MET | GLY | LEU | VAL | ||||
| 7 | ASN | ALA | ILE | PRO | MET | ILE | ALA | VAL | GLY | LEU | ||||
| 8 | GLY | LEU | TYR | VAL | MET | PHE | ALA | VAL | ALA |
Samples:
sample_1: subunit c, [U-98% 13C; U-98% 15N], 2.0 mM; CDCl3 44.4 % v/v; CD3OH 44.4 % v/v; H20 11.1 % v/v
Ex-cond_1: pH: 5.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HCACO | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HC(CO)NH | not available | not available | not available |
| TOCSY-HSQC | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C-13C NOESY | not available | not available | not available |
| 1H-13C-15N NOESY | not available | not available | not available |
| NOE-HNCO | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- BRUKER DMX 500 MHz
Related Database Links:
| BMRB | 10021 15564 4146 4151 |
| PDB | |
| DBJ | BAB38102 BAE75684 BAE77551 BAG79551 BAH65925 |
| EMBL | CAA23515 CAA23522 CAA24752 CAA25777 CAD03125 |
| GB | AAA23668 AAA24732 AAA62089 AAA83870 AAC76760 |
| PIR | AI0953 |
| REF | NP_290376 NP_312706 NP_418193 NP_458073 NP_462769 |
| SP | A4TSI8 A4WGF0 A6TG41 A7FPE5 A7MMV9 |
| AlphaFold | A7FPE5 A4TSI8 A4WGF0 A6TG41 A7MMV9 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks