BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5331

Title: Backbone relaxation data of rat holo cellular retinol-binding protein type I (CRBP-I)   PubMed: 11934897

Authors: Franzoni, Lorella; Luecke, Christian; Perez, Carlos; Cavazzini, Davide; Rademacher, Martin; Ludwig, Christian; Spisni, Alberto; Rossi, Gian Luigi; Rueterjans, Heinz

Citation: Franzoni, Lorella; Luecke, Christian; Perez, Carlos; Cavazzini, Davide; Rademacher, Martin; Ludwig, Christian; Spisni, Alberto; Rossi, Gian Luigi; Rueterjans, Heinz. "Structure and backbone dynamics of apo- and holo-cellular retinol-binding protein in solution"  J. Biol. Chem. 277, 21983-21997 (2002).

Assembly members:
cellular retinol-binding protein type I, polymer, 135 residues, 15703 Da.
RTL, non-polymer, 286.452 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cellular retinol-binding protein type I: MPVDFNGYWKMLSNENFEEY LRALDVNVALRKIANLLKPD KEIVQDGDHMIIRTLSTFRN YIMDFQVGKEFEEDLTGIDD RKCMTTVSWDGDKLQCVQKG EKEGRGWTQWIEGDELHLEM RAEGVTCKQVFKKVH

Data sets:
Data typeCount
T1 relaxation values235
T2 relaxation values236
order parameters118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cellular retinol-binding protein type I1
2retinol2

Entities:

Entity 1, cellular retinol-binding protein type I 135 residues - 15703 Da.

1   METPROVALASPPHEASNGLYTYRTRPLYS
2   METLEUSERASNGLUASNPHEGLUGLUTYR
3   LEUARGALALEUASPVALASNVALALALEU
4   ARGLYSILEALAASNLEULEULYSPROASP
5   LYSGLUILEVALGLNASPGLYASPHISMET
6   ILEILEARGTHRLEUSERTHRPHEARGASN
7   TYRILEMETASPPHEGLNVALGLYLYSGLU
8   PHEGLUGLUASPLEUTHRGLYILEASPASP
9   ARGLYSCYSMETTHRTHRVALSERTRPASP
10   GLYASPLYSLEUGLNCYSVALGLNLYSGLY
11   GLULYSGLUGLYARGGLYTRPTHRGLNTRP
12   ILEGLUGLYASPGLULEUHISLEUGLUMET
13   ARGALAGLUGLYVALTHRCYSLYSGLNVAL
14   PHELYSLYSVALHIS

Entity 2, retinol - C20 H30 O - 286.452 Da.

1   RTL

Samples:

sample_1: cellular retinol-binding protein type I, [U-15N], 1.6 mM; RETINOL 1.6 mM

cond_set_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N T1sample_1not availablecond_set_1
15N T2sample_1not availablecond_set_1
{1H}-15N NOEsample_1not availablecond_set_1

Software:

XWINNMR v1.3 - data acquisition, data processing

RMX vrev. II/1997 - data analysis

MODELFREE v4.01 - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 5048 5319 5330 5578 5579
PDB
DBJ BAB27753
EMBL CAA42919
GB AAA35714 AAA40962 AAA42021 AAH18254 AAH91751
REF NP_035384 NP_036865 XP_005080789 XP_005366741 XP_006083819
SP P02696 Q00915