BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5332

Title: 1H, 13C, and 15N Chemical Shift Assignments for E85Q recoverin   PubMed: 11980481

Authors: Ames, James; Hamasaki, N.; Molchanova, T.

Citation: Ames, James; Hamasaki, N.; Molchanova, T.. "Structure and Calcium-binding Studies of a Recoverin Mutant (E85Q) in an Allosteric Intermediate State"  Biochemistry 41, 5776-5787 (2002).

Assembly members:
Recoverin, polymer, 202 residues, 23333.4 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Recoverin: XGNSKSGALSKEILEELQLN TKFTEEELSSWYQSFLKECP SGRITRQEFQTIYSKFFPEA DPKAYAQHVFRSFDANSDGT LDFKEYVIALHMTSAGKTNQ KLEWAFSLYDVDGNGTISKN EVLEIVTAIFKMISPEDTKH LPEDENTPEKRAEKIWGFFG KKDDDKLTEKEFIEGTLANK EILRLIQFEPQKVKEKLKEK KL

Data sets:
Data typeCount
1H chemical shifts540
13C chemical shifts523
15N chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1recoverin mutant E85Q1
2Ca++, I2
3Ca++, II2

Entities:

Entity 1, recoverin mutant E85Q 202 residues - 23333.4 Da.

1   MYRGLYASNSERLYSSERGLYALALEUSER
2   LYSGLUILELEUGLUGLULEUGLNLEUASN
3   THRLYSPHETHRGLUGLUGLULEUSERSER
4   TRPTYRGLNSERPHELEULYSGLUCYSPRO
5   SERGLYARGILETHRARGGLNGLUPHEGLN
6   THRILETYRSERLYSPHEPHEPROGLUALA
7   ASPPROLYSALATYRALAGLNHISVALPHE
8   ARGSERPHEASPALAASNSERASPGLYTHR
9   LEUASPPHELYSGLUTYRVALILEALALEU
10   HISMETTHRSERALAGLYLYSTHRASNGLN
11   LYSLEUGLUTRPALAPHESERLEUTYRASP
12   VALASPGLYASNGLYTHRILESERLYSASN
13   GLUVALLEUGLUILEVALTHRALAILEPHE
14   LYSMETILESERPROGLUASPTHRLYSHIS
15   LEUPROGLUASPGLUASNTHRPROGLULYS
16   ARGALAGLULYSILETRPGLYPHEPHEGLY
17   LYSLYSASPASPASPLYSLEUTHRGLULYS
18   GLUPHEILEGLUGLYTHRLEUALAASNLYS
19   GLUILELEUARGLEUILEGLNPHEGLUPRO
20   GLNLYSVALLYSGLULYSLEULYSGLULYS
21   LYSLEU

Entity 2, Ca++, I - Ca - 40.078 Da.

1   CA

Samples:

sample1: Recoverin, [U-13C; U-15N], 0.8 mM

Ex-cond_1: pH: 6.7; temperature: 310 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample1not availableEx-cond_1
1H-15N TOCSYsample1not availableEx-cond_1
HNCACBsample1not availableEx-cond_1
HNCOsample1not availableEx-cond_1
CBCACONHsample1not availableEx-cond_1
HBHACONHsample1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4030
PDB
EMBL CAA44928
GB AAB59256 ELR47741
PRF 1802271A
REF NP_776590 XP_004012748 XP_005693626 XP_005907603 XP_005959860
SP P21457
TPG DAA18803