BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5333

Title: Redox-Coupled Conformational Alternations in Cytochrome c3 from D. vulgaris Miyazaki F on the Basis of its Reduced Solution Structure   PubMed: 12054869

Authors: Harada, Erisa; Fukuoka, Yuki; Ohmura, Tomoaki; Fukunishi, Arima; Kawai, Gota; Fujiwara, Toshimichi; Akutsu, Hideo

Citation: Harada, Erisa; Fukuoka, Yuki; Ohmura, Tomoaki; Fukunishi, Arima; Kawai, Gota; Fujiwara, Toshimichi; Akutsu, Hideo. "Redox-coupled Conformational Alternations in Cytochrome c3 from D. vulgaris Miyazaki F on the Basis of its Reduced Solution Structure"  J. Mol. Biol. 319, 767-778 (2002).

Assembly members:
cytochrome c3, polymer, 107 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: not available   Taxonomy ID: 883   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio vulgaris

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
cytochrome c3: APKAPADGLKMDKTKQPVVF NHSTHKAVKCGDCHHPVNGK EDYQKCATAGCHDNMDKKDK SAKGYYHAMHDKGTKFKSCV GCHLETAGADAAKKKELTGC KGSKCHS

Data sets:
Data typeCount
1H chemical shifts782
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c3 peptide1
2heme 12
3heme 22
4heme 32
5heme 42

Entities:

Entity 1, cytochrome c3 peptide 107 residues - Formula weight is not available

1   ALAPROLYSALAPROALAASPGLYLEULYS
2   METASPLYSTHRLYSGLNPROVALVALPHE
3   ASNHISSERTHRHISLYSALAVALLYSCYS
4   GLYASPCYSHISHISPROVALASNGLYLYS
5   GLUASPTYRGLNLYSCYSALATHRALAGLY
6   CYSHISASPASNMETASPLYSLYSASPLYS
7   SERALALYSGLYTYRTYRHISALAMETHIS
8   ASPLYSGLYTHRLYSPHELYSSERCYSVAL
9   GLYCYSHISLEUGLUTHRALAGLYALAASP
10   ALAALALYSLYSLYSGLULEUTHRGLYCYS
11   LYSGLYSERLYSCYSHISSER

Entity 2, heme 1 - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample: cytochrome c3, [U-15N], 1.0 – 2.0 mM

sample_conditon: pH: 7.0 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsamplenot availablesample_conditon
1H-15N TOCSYsamplenot availablesample_conditon
NOESYsamplenot availablesample_conditon
DQF-COSYsamplenot availablesample_conditon
E-COSYsamplenot availablesample_conditon

Software:

NMR-Pipe/Pipp v4.0 -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AMX 500 MHz
  • Bruker DRX 400 MHz

Related Database Links:

BMRB 6572
PDB
DBJ BAA06511
GB ACL09439 EGY24874
PRF 0607221A 1916446A
REF WP_007526318 WP_015946129 YP_002436907
SP P00132