BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5337

Title: Complete assignments of 1H, 13C and 15N Chemical Shifts for Oxidized Human Adrenodoxin (4-114)

Authors: Kostic, Milka; Pochapsky, Susan; Obenauer, John; Mo, Huaping; Pagani, Gina; Pejchal, Robert; Pochapsky, Thomas

Citation: Kostic, Milka; Pochapsky, Susan; Obenauer, John; Mo, Huaping; Pagani, Gina; Pejchal, Robert; Pochapsky, Thomas. "Comparison of functional domains in vertebrate-type ferredoxins"  Biochemistry ., .-..

Assembly members:
human adrenodoxin, polymer, 111 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
human adrenodoxin: EDKITVHFINRDGETLTTKG KVGDSLLDVVVENNLDIDGF GACDGTLACSTCHLIFEDHI YEKLDAITDEENDMLDLAYG LTDRSRLGCQICLTKSMDNM TVRVPETVADA

Data sets:
Data typeCount
1H chemical shifts578
13C chemical shifts374
15N chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human Adx (4-114), chain A1
2human Adx (4-114), chain B1
3FES A2
4FES B2

Entities:

Entity 1, human Adx (4-114), chain A 111 residues - Formula weight is not available

1   GLUASPLYSILETHRVALHISPHEILEASN
2   ARGASPGLYGLUTHRLEUTHRTHRLYSGLY
3   LYSVALGLYASPSERLEULEUASPVALVAL
4   VALGLUASNASNLEUASPILEASPGLYPHE
5   GLYALACYSASPGLYTHRLEUALACYSSER
6   THRCYSHISLEUILEPHEGLUASPHISILE
7   TYRGLULYSLEUASPALAILETHRASPGLU
8   GLUASNASPMETLEUASPLEUALATYRGLY
9   LEUTHRASPARGSERARGLEUGLYCYSGLN
10   ILECYSLEUTHRLYSSERMETASPASNMET
11   THRVALARGVALPROGLUTHRVALALAASP
12   ALA

Entity 2, FES A - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: human adrenodoxin, [U-90% 13C; U-90% 15N], 1.5 mM

Experimental_Conditions: pH: 7.4; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableExperimental_Conditions
HNCAsample_1not availableExperimental_Conditions
HN(CO)CAsample_1not availableExperimental_Conditions
HNCOsample_1not availableExperimental_Conditions
HNCACBsample_1not availableExperimental_Conditions
HCCH-TOCSYsample_1not availableExperimental_Conditions
1H-15N HSQCsample_1not availableExperimental_Conditions
1H-13C HSQCsample_1not availableExperimental_Conditions

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 4073 4074 4439 4440 6026
PDB
DBJ BAG73145
GB AAA35829 AAA35855 AAA35856 AAA50462 AAA76853
REF NP_004100 XP_001105034 XP_003253520 XP_003910717 XP_003923949
SP P10109