BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5338

Title: Integrin EGF-like module 3 from the beta-2 subunit   PubMed: 11896403

Authors: Beglova, N.; Blacklow, S.; Takagi, J.; Springer, T.

Citation: Beglova, N.; Blacklow, S.; Takagi, J.; Springer, T.. "Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation "  Nat. Struct. Biol. 9, 282-287 (2002).

Assembly members:
integrin, polymer, 41 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
integrin: ECDTINCERYNGQVCGGPGR GLCFCGKCRCHPGFEGSACQ A

Data sets:
Data typeCount
1H chemical shifts232
15N chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IEGF-31

Entities:

Entity 1, IEGF-3 41 residues - Formula weight is not available

1   GLUCYSASPTHRILEASNCYSGLUARGTYR
2   ASNGLYGLNVALCYSGLYGLYPROGLYARG
3   GLYLEUCYSPHECYSGLYLYSCYSARGCYS
4   HISPROGLYPHEGLUGLYSERALACYSGLN
5   ALA

Samples:

sample_1: integrin, [U-15N], 1 mM; NaPO4 5 mM; H2O 90%; D2O 10%

sample_2: integrin 1 mM; NaPO4 5 mM; D2O 100%

sample_cond_1: pH: 6.2; temperature: 298 K; ionic strength: 5 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
HMQC-Jnot availablenot availablenot available
1H-NOESYnot availablenot availablenot available
1H-TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

GIFA v4.3 - processing

XEASY v1.3.13 - data analysis

CNS v1.0 - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAD96225 BAG53190 BAG64523 BAI45957
EMBL CAA45427 CAA68266 CAB90553
GB AAA59490 AAH05861 AAH21077 AAP88748 AAQ14924
REF NP_000202 NP_001029294 NP_001120963 NP_001290167 XP_003823870
SP P05107