BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5348

Title: Solution structure of ascidian trypsin inhibitor determined by nuclear magnetic resonance spectroscopy

Authors: Hemmi, Hikaru; Yoshida, Takuya; Kumazaki, Takashi; Nemoto, Nobuaki; Hasegawa, Jun; Nishioka, Fujio; Kyogoku, Yoshimasa; Yokosawa, Hideyoshi; Kobayashi, Yuji

Citation: Hemmi, Hikaru; Yoshida, Takuya; Kumazaki, Takashi; Nemoto, Nobuaki; Hasegawa, Jun; Nishioka, Fujio; Kyogoku, Yoshimasa; Yokosawa, Hideyoshi; Kobayashi, Yuji. "Solution structure of ascidian trypsin inhibitor determined by nuclear magnetic resonance spectroscopy"  Biochemistry 41, 10657-10664 (2002).

Assembly members:
ascidian trypsin inhibitor, polymer, 55 residues, Formula weight is not available

Natural source:   Common Name: Halocynthia roretzi   Taxonomy ID: 7729   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Halocynthia roretzi

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
ascidian trypsin inhibitor: AHMDCTEFNPLCRCNKMLGD LICAVIGDAKEEHRNMCALC CEHPGGFEYSNGPCE

Data sets:
Data typeCount
1H chemical shifts349

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ati monomer1

Entities:

Entity 1, ati monomer 55 residues - Formula weight is not available

1   ALAHISMETASPCYSTHRGLUPHEASNPRO
2   LEUCYSARGCYSASNLYSMETLEUGLYASP
3   LEUILECYSALAVALILEGLYASPALALYS
4   GLUGLUHISARGASNMETCYSALALEUCYS
5   CYSGLUHISPROGLYGLYPHEGLUTYRSER
6   ASNGLYPROCYSGLU

Samples:

sample_1: ascidian trypsin inhibitor1.5 – 2.0 mM

Ex-cond_1: pH: 3.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQFCOSYnot availablenot availablenot available
1H-1H ECOSYnot availablenot availablenot available

Software:

NMRPipe - processing

PIPP - assignment, peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz
  • JEOL alpha 600 MHz

Related Database Links:

PDB
SP P16589