BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5353

Title: 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state

Authors: Ishida, Hiroaki

Citation: Ishida, Hiroaki; Nakashima, K-i; Kumaki, Yasuhiro; Nakata, Mitsuo; Hikichi, Kunio; Yazawa, Michio. "Letter to the Editor: 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state"  J. Biomol. NMR 23, 323-324 (2002).

Assembly members:
yeast calmodulin, polymer, 146 residues, 16000 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
yeast calmodulin: SSNLTEEQIAEFKEAFALFD KDNNGSISSSELATVMRSLG LSPSEAEVNDLMNEIDVDGN HQIEFSEFLALMSRQLKSND SEQELLEAFKVFDKNGDGLI SAAELKHVLTSIGEKLTDAE VDDMLREVSDGSGEINIQQF AALLSK

Data sets:
Data typeCount
1H chemical shifts142
15N chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yCaM monomer1

Entities:

Entity 1, yCaM monomer 146 residues - 16000 Da.

1   SERSERASNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHEALALEUPHEASP
3   LYSASPASNASNGLYSERILESERSERSER
4   GLULEUALATHRVALMETARGSERLEUGLY
5   LEUSERPROSERGLUALAGLUVALASNASP
6   LEUMETASNGLUILEASPVALASPGLYASN
7   HISGLNILEGLUPHESERGLUPHELEUALA
8   LEUMETSERARGGLNLEULYSSERASNASP
9   SERGLUGLNGLULEULEUGLUALAPHELYS
10   VALPHEASPLYSASNGLYASPGLYLEUILE
11   SERALAALAGLULEULYSHISVALLEUTHR
12   SERILEGLYGLULYSLEUTHRASPALAGLU
13   VALASPASPMETLEUARGGLUVALSERASP
14   GLYSERGLYGLUILEASNILEGLNGLNPHE
15   ALAALALEULEUSERLYS

Samples:

sample_1: yeast calmodulin, [U-13C; U-15N], 1.0 mM; yeast calmodulin, [U-13C; U-15N], 1.4 mM

sample_2: yeast calmodulin, [U-15N], 1.0 mM; yeast calmodulin, [U-15N], 1.4 mM

experimental_conditions: pH: 7.0; temperature: 303 K; ionic strength: 0.05 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

XEASY - peak assignments

NMR spectrometers:

  • Varian UNITY 500 MHz
  • JEOL JNM 600 MHz

Related Database Links:

BMRB 17850
PDB
DBJ GAA21657
EMBL CAA55612 CAA85064 CAG58389 CAR27417 CBK39182
GB AAA34504 AAC68888 AAS56510 AHY74589 EDN64723
REF NP_009667 XP_001642859 XP_002496350 XP_003678123 XP_003680602
SP P06787
TPG DAA07228