BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5361

Title: Solution Structure of the DNA Complex of Human TRF1   PubMed: 11738049

Authors: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.

Citation: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.. "Solution Structure of a Telomeric DNA Complex of Human TRF1"  Structure 9, 1237-1251 (2001).

Assembly members:
Telomeric repeat binding factor 1, polymer, 70 residues, Formula weight is not available
none, polymer, 13 residues, Formula weight is not available
none, polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Telomeric repeat binding factor 1: MTPEKHRARKRQAWLWEEDK NLRSGVRKYGEGNWSKILLH YKFNNRTSVMLKDRWRTMKK LKLISSDSED
none: GTTAGGGTTAGGG
none: CCCTAACCCTAAC

Data typeCount
1H chemical shifts655
13C chemical shifts186
15N chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TELOMERE REPEAT BINDING FACTOR 11
25'-D(*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3'2
35'-D(*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*C)-3'3

Entities:

Entity 1, TELOMERE REPEAT BINDING FACTOR 1 70 residues - Formula weight is not available

1   METTHRPROGLULYSHISARGALAARGLYS
2   ARGGLNALATRPLEUTRPGLUGLUASPLYS
3   ASNLEUARGSERGLYVALARGLYSTYRGLY
4   GLUGLYASNTRPSERLYSILELEULEUHIS
5   TYRLYSPHEASNASNARGTHRSERVALMET
6   LEULYSASPARGTRPARGTHRMETLYSLYS
7   LEULYSLEUILESERSERASPSERGLUASP

Entity 2, 5'-D(*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3' 13 residues - Formula weight is not available

1   DGDTDTDADGDGDGDTDTDA
2   DGDGDG

Entity 3, 5'-D(*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*C)-3' 13 residues - Formula weight is not available

1   DCDCDCDTDADADCDCDCDT
2   DADADC

Samples:

sample_1: Telomeric repeat binding factor 1, [U-15N; U-13C], 1.5 – 2.5 mM; none1.5 – 2.5 mM; none1.5 – 2.5 mM; Phosphate buffer 5 mM; NaCl 10 mM

sample_cond_1: pH: 6.8; temperature: 305 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1not availablesample_cond_1
3D HN(CO)CAsample_1not availablesample_cond_1
3D CBCA(CO)NHsample_1not availablesample_cond_1
3D C(CO)NHsample_1not availablesample_cond_1
3D HBHA(CO)NHsample_1not availablesample_cond_1
3D HCCH-TOCSYsample_1not availablesample_cond_1
3D HNHAsample_1not availablesample_cond_1
3D HNHBsample_1not availablesample_cond_1
3D 13C-NOESYsample_1not availablesample_cond_1
3D 15N-NOESYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D COSY with or without isotope filteringsample_1not availablesample_cond_1

Software:

EMBOSS v5.0 - refinement, structure solution

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4210 5317
PDB
DBJ BAF85218
EMBL CAA63768 CAH92488
GB AAB53363 AAB54036 AAB81137 AAC02531 AAH29378
REF NP_001126467 NP_003209 NP_059523 XP_001164723 XP_001164763
SP O55036 P54274