BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5363

Title: 1H, 13C, and 15N Chemical shifts for hERR2 Protein, 1H chemical shifts for DNA   PubMed: 12654265

Authors: Gearhart, Micah; Holmbeck, Signe; Evans, Ronald; Dyson, H.; Wright, Peter

Citation: Gearhart, Micah; Holmbeck, Signe; Evans, Ronald; Dyson, H.; Wright, Peter. "Monomeric Complex of Human Orphan Estrogen Related Receptor 2 with DNA: A Pseudo-dimer Interface Mediates Extended Half-site Recognition "  J. Mol. Biol. 327, 819-832 (2003).

Assembly members:
Estrogen Related Receptor 2, polymer, 98 residues, Formula weight is not available
DNA Strand1, polymer, 13 residues, Formula weight is not available
DNA Strand2, polymer, 13 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Estrogen Related Receptor 2: AIPKRLCLVCGDIASGYHYG VASCEACKAFFKRTIQGNIE YSCPATNECEITKRRRKSCQ ACRFMKALKVGMLKEGVRLD RVRGGRQKYKRRLDSENS
DNA Strand1: GCTCAAGGTCACG
DNA Strand2: CGTGACCTTGAGC

Data sets:
Data typeCount
1H chemical shifts846
13C chemical shifts322
15N chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hERR2 protein1
2DNA strand 12
3DNA strand 23
4ZINC (II) ION4

Entities:

Entity 1, hERR2 protein 98 residues - Formula weight is not available

1   ALAILEPROLYSARGLEUCYSLEUVALCYS
2   GLYASPILEALASERGLYTYRHISTYRGLY
3   VALALASERCYSGLUALACYSLYSALAPHE
4   PHELYSARGTHRILEGLNGLYASNILEGLU
5   TYRSERCYSPROALATHRASNGLUCYSGLU
6   ILETHRLYSARGARGARGLYSSERCYSGLN
7   ALACYSARGPHEMETLYSALALEULYSVAL
8   GLYMETLEULYSGLUGLYVALARGLEUASP
9   ARGVALARGGLYGLYARGGLNLYSTYRLYS
10   ARGARGLEUASPSERGLUASNSER

Entity 2, DNA strand 1 13 residues - Formula weight is not available

1   DGDCDTDCDADADGDGDTDC
2   DADCDG

Entity 3, DNA strand 2 13 residues - Formula weight is not available

1   DCDGDTDGDADCDCDTDTDG
2   DADGDC

Entity 4, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: Estrogen Related Receptor 2, [U-13C; U-15N], 0.5 mM; DNA Strand1 0.5 mM; DNA Strand2 0.5 mM; ZINC (II) ION 0.5 mM; K2SO4 20 mM; ZnSO4 50 mM; dithiothreitol (DTT) 2 mM; NaN3 0.001%

Sample_2: Estrogen Related Receptor 2, [U-15N], 0.5 mM; DNA Strand1 0.5 mM; DNA Strand2 0.5 mM; ZINC (II) ION 0.5 mM; K2SO4 20 mM; ZnSO4 50 mM; dithiothreitol (DTT) 2 mM; NaN3 0.001%

Sample_3: Estrogen Related Receptor 2, [U-15N], 0.5 mM; K2SO4 20 mM; ZnSO4 50 mM; dithiothreitol (DTT) 2 mM; NaN3 0.001%

sample_4: DNA Strand1 0.5 mM; DNA Strand2 0.5 mM; K2SO4 20 mM; ZnSO4 50 mM; dithiothreitol (DTT) 2 mM; NaN3 0.001%

cond1: pH: 6.8; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablecond1
HNCOCAnot availablenot availablecond1
HNCOnot availablenot availablecond1
HCACOnot availablenot availablecond1
(H)CCH-COSYnot availablenot availablecond1
(H)CCH TOCSYnot availablenot availablecond1
HNHAnot availablenot availablecond1
HNHBnot availablenot availablecond1
HA(CA)HB-COSYnot availablenot availablecond1
2D [13C,15N ] u2-filtered TOCSYnot availablenot availablecond1
2D [13C,15N] u1,u2-filtered NOESYnot availablenot availablecond1
3D 15N separated NOESYnot availablenot availablecond1
3D 13C-separated NOESYnot availablenot availablecond1

Software:

NMRView v4.2 - spectral analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 4033 4034
PDB
DBJ BAC27656 BAC34898 BAG73125 BAH02305
EMBL CAA35779 CAA61755 CAG09135 CDQ87849
GB AAB37687 AAC99409 AAG29619 AAH44858 AAI11278
PIR B29345
PRF 1402310B
REF NP_001008516 NP_001152972 NP_001156563 NP_004443 NP_036064
SP O95718 P11475 Q61539
TPG DAA24995