BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5365

Title: 1H, 15N, 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor

Authors: Chuang, Woei-Jer

Citation: Chuang, Woei-Jer; Yeh, I-Ju; Hsieh, Yu-Huei; Liu, Pei-Phen; Chen, Shu-Wan; Jeng, W.-Y.. "Letters to the Editor: 1H, 15N, 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor (Foxk1)"  J. Biomol. NMR 24, 75-76 (2002).

Assembly members:
myocyte nuclear factor, polymer, 114 residues, 13012 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
myocyte nuclear factor: ASMTGGQQMGRGSESKPPYS YAQLIVQAISSAQDRQLTLS GIYAHITKHYPYYRTADKGW QNSIRHNLSLNRYFIKVPRS QEEPGKGSFWRIDPASEAKL VEQAFRKRRQRGVS

Data sets:
Data typeCount
1H chemical shifts661
13C chemical shifts184
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myocyte nuclear factor1

Entities:

Entity 1, myocyte nuclear factor 114 residues - 13012 Da.

1   ALASERMETTHRGLYGLYGLNGLNMETGLY
2   ARGGLYSERGLUSERLYSPROPROTYRSER
3   TYRALAGLNLEUILEVALGLNALAILESER
4   SERALAGLNASPARGGLNLEUTHRLEUSER
5   GLYILETYRALAHISILETHRLYSHISTYR
6   PROTYRTYRARGTHRALAASPLYSGLYTRP
7   GLNASNSERILEARGHISASNLEUSERLEU
8   ASNARGTYRPHEILELYSVALPROARGSER
9   GLNGLUGLUPROGLYLYSGLYSERPHETRP
10   ARGILEASPPROALASERGLUALALYSLEU
11   VALGLUGLNALAPHEARGLYSARGARGGLN
12   ARGGLYVALSER

Samples:

sample_1: myocyte nuclear factor, [U-15N; U-13C], 3 mM; phosphate buffer 25 mM; NaCl 100 mM

sample_2: myocyte nuclear factor, [U-15N], 3 mM; phosphate buffer 25 mM; NaCl 100 mM

sample_3: myocyte nuclear factor, [15N]-Ala, 1 mM; phosphate buffer 25 mM; NaCl 100 mM

sample_4: myocyte nuclear factor 3 mM; phosphate buffer 25 mM; NaCl 100 mM

condition_1: pH: 6.0; temperature: 300 K; ionic strength: 1.5 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CACB(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HBHA(CBCA)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HCC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available

Software:

aurelia v2.7.5 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAE25880 BAE27871 BAG53651 BAG63595
GB AAA37529 AAB69641 AAF97842 AAH54664 AAI56274
REF NP_001032242 NP_001032296 NP_951031 NP_956196 XP_002723111
SP P42128 P85037
TPG DAA15159