BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5366

Title: SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN   PubMed: 11993989

Authors: Lu, J.; Garcia, .; Dulubova, I.; Sudhof, T.; Rizo, J.

Citation: Lu, J.; Garcia, J.; Dulubova, I.; Sudhof, T.; Rizo, J.. "Solution Structure of the Vam7P PX Domain"  Biochemistry 41, 5956-5962 (2002).

Assembly members:
vam7p, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
vam7p: KMSEKLRKMSEKLRIKVDDV KINPKYVLYGVSTPNKRLYK RYSEFWKLKTRLERDVGSTI PYDFPEKPGVLDRRWQRRYD DPEMIDERRIGLERFLNELY NDRFDSRWRDTKIAQDFLQL SKPN

Data sets:
Data typeCount
13C chemical shifts575
15N chemical shifts127
1H chemical shifts897

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vacuolar morphogenesis protein1

Entities:

Entity 1, Vacuolar morphogenesis protein 124 residues - Formula weight is not available

1   LYSMETSERGLULYSLEUARGLYSMETSER
2   GLULYSLEUARGILELYSVALASPASPVAL
3   LYSILEASNPROLYSTYRVALLEUTYRGLY
4   VALSERTHRPROASNLYSARGLEUTYRLYS
5   ARGTYRSERGLUPHETRPLYSLEULYSTHR
6   ARGLEUGLUARGASPVALGLYSERTHRILE
7   PROTYRASPPHEPROGLULYSPROGLYVAL
8   LEUASPARGARGTRPGLNARGARGTYRASP
9   ASPPROGLUMETILEASPGLUARGARGILE
10   GLYLEUGLUARGPHELEUASNGLULEUTYR
11   ASNASPARGPHEASPSERARGTRPARGASP
12   THRLYSILEALAGLNASPPHELEUGLNLEU
13   SERLYSPROASN

Samples:

sample_1: vam7p, [U-15N], 0.5 mM; Mes buffer 20 mM; H2O 90%; D2O 10%

sample_2: vam7p, [U-15N; U-13C], 0.5 mM; Mes buffer 20 mM; H2O 90%; D2O 10%

sample_3: vam7p, [U-15N], 0.5 mM; Mes buffer 20 mM; D2O 100%

sample_4: vam7p, [U-10% 13C], 0.5 mM; Mes buffer 20 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 20 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

NMRPipe v1.8 - processing

VNMR v6.1 - collection

NMRView v4.1.2 - data analysis

CNS v1.0 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB EGA86963