BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5369

Title: A New Zinc Binding Fold Underlines the Versatility of Zinc Binding Modules in Protein Evolution   PubMed: 12015147

Authors: Sharpe, B.; Matthews, J.; Kwan, A.; Newton, A.; Gell, D.; Crossley, M.; Mackay, J.

Citation: Sharpe, B.; Matthews, J.; Kwan, A.; Newton, A.; Gell, D.; Crossley, M.; Mackay, J.. "A New Zinc Binding Fold Underlines the Versatility of Zinc Binding Modules in Protein Evolution"  Structure 10, 636-648 (2002).

Assembly members:
CREB Binding Protein, polymer, 27 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CREB Binding Protein: EVRACSLPHCRTMKNVLNHM THCQAGK

Data sets:
Data typeCount
1H chemical shifts168
coupling constants10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CREB Binding Protein1
2Zinc ion2

Entities:

Entity 1, CREB Binding Protein 27 residues - Formula weight is not available

1   GLUVALARGALACYSSERLEUPROHISCYS
2   ARGTHRMETLYSASNVALLEUASNHISMET
3   THRHISCYSGLNALAGLYLYS

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CREB Binding Protein 0.4 mM; ZINC (II) ION 0.6 mM; TCEP 0.6 mM

sample_cond_1: pH: 6.5; temperature: 275 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - data analysis

CNS v1.0 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

REF XP_001095225 NP_004371 NP_596872 NP_001020603 NP_001073315
SWISS-PROT Q92793
GenBank AAH72594 AAL87531 AAC51331 AAC51770 AAC08447
EMBL CAF96470
DBJ BAE06125 BAG65526
PDB
BMRB 6268 5327 5987