BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5379

Title: Bovine Pancreatic Polypeptide Bound to DPC Micelles   PubMed: 12367532

Authors: Lerch, Mirjam; Gafner, Verena; Bader, Reto; Christen, Barbara; Zerbe, Oliver

Citation: Lerch, Mirjam; Gafner, Verena; Bader, Reto; Christen, Barbara; Folkers, Gerd; Zerbe, Oliver. "Bovine Pancreatic Polypeptide (bPP) undergoes Significant changes in Conformation and Dynamics upon Binding to DPC Micelles "  J. Mol. Biol. 322, 1117-1133 (2002).

Assembly members:
Pancreatic polypeptide, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Pancreatic polypeptide: APLEPEYPGDNATPEQMAQY AAELRRYINMLTRPRYX

Data sets:
Data typeCount
1H chemical shifts257
15N chemical shifts30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pancreatic polypeptide1

Entities:

Entity 1, pancreatic polypeptide 37 residues - Formula weight is not available

1   ALAPROLEUGLUPROGLUTYRPROGLYASP
2   ASNALATHRPROGLUGLNMETALAGLNTYR
3   ALAALAGLULEUARGARGTYRILEASNMET
4   LEUTHRARGPROARGTYRNH2

Samples:

sample_1: Pancreatic polypeptide, [U-95% 15N], 3 mM; DPC-d38 300 mM

sample_cond_1: pH*: 5.5; temperature: 310 K; ionic strength: 0 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.6 - processing

XEASY v1.53 - data analysis

DYANA v1.5 - structure solution

AMBER v6 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 2223 5548
PDB
GB AAA98526 AAR11453 EFB29809 ELK14152
REF NP_776377 XP_002919776 XP_003997019 XP_004013021 XP_004286056
SP P01301 P01302 P06884 P39659
TPG DAA18335