BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5385

Title: Chemical shift assignments for the 8OG:G mismatched duplex   PubMed: 12498794

Authors: Thiviyanathan, Varatharasa; Somasunderam, Anoma; Hazra, Tapas; Mitra, Sankar; Gorenstein, David

Citation: Thiviyanathan, Varatharasa; Somasunderam, Anoma; Hazra, Tapas; Mitra, Sankar; Gorenstein, David. "Solution Structure of a DNA Duplex containing 8-hydroxy-2'-deoxyguanosine Opposite Deoxyguanosine"  J. Mol. Biol. 325, 433-442 (2003).

Assembly members:
DNA, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA: CGCGAATTGGCG

Data sets:
Data typeCount
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
18-OG 11
28-OG 21

Entities:

Entity 1, 8-OG 1 12 residues - Formula weight is not available

1   DCDGDCDGDADADTDTDGDG
2   DCDG

Samples:

sample_1: DNA1.8 – 2.2 mM

condition_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablecondition_1
DQF-COSYsample_1not availablecondition_1
2D-TOCSYsample_1not availablecondition_1
31P-1H HETCORsample_1not availablecondition_1
HEHAHAsample_1not availablecondition_1

Software:

AMBER v6.0 - Molecular Dynamics

NMR spectrometers:

  • Varian Unity-Plus 750 MHz
  • Varian Unity-Plus 600 MHz

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