BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5395

Title: Chemical Shift Assignments for the Unmodified Branch Site Helix   PubMed: 12426583

Authors: Newby, Meredith; Greenbaum, Nancy

Citation: Newby, Meredith; Greenbaum, Nancy. "Sculpting of the Spliceosomal Branch Site Recognition Motif by a Conserved Pseudouridine"  Nat. Struct. Biol. 9, 958-965 (2002).

Assembly members:
U2 snRNA - intron duplex, polymer, 9 residues, Formula weight is not available
Intron branch site region, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
U2 snRNA - intron duplex: GGUGUAGUA
Intron branch site region: UACUAACACC

Data sets:
Data typeCount
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Unmodified U2 snRNA strand1
2Intron strand2

Entities:

Entity 1, Unmodified U2 snRNA strand 9 residues - Formula weight is not available

1   GGUGUAGUA

Entity 2, Intron strand 10 residues - Formula weight is not available

1   UACUAACACC

Samples:

sample_1: U2 snRNA - intron duplex 1.0 mM; Intron branch site region 1.0 mM; sodium phosphate 10 mM; D2O 10%; H2O 90%; EDTA 0.1 mM

sample_2: U2 snRNA - intron duplex 1.0 mM; Intron branch site region 1.0 mM; sodium phosphate 10 mM; D2O 100%; EDTA 0.1 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 278 K

sample_cond_2: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

VNMR v5.3 - collection

SPARKY v3.69 - data analysis

X-PLOR v3.851 - refinement, structure solution

NMR spectrometers:

  • Varian UNITYplus 720 MHz

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