BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5403

Title: Backbone resonance assignments of the 91 kDa oligomeric TRAP protein from Bacillus staerothermophilus in complex with L-tryptophan

Authors: McElroy, Craig; Manfredo, Amanda; Wendt, Alice; Gollnick, Paul; Foster, Mark

Citation: McElroy, Craig; Manfredo, Amanda; Wendt, Alice; Gollnick, Paul; Foster, Mark. "TROSY-NMR Studies of the 91 kDa TRAP Protein reveal Allosteric Control of a Gene Regulatory Protein by Ligand-altered Flexibility"  J. Mol. Biol. 323, 463-473 (2002).

Assembly members:
trp RNA-binding attenuation protein, polymer, 74 residues, 8243 Da.
TRYPTOPHAN, non-polymer, 204.225 Da.

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
trp RNA-binding attenuation protein: MYTNSDFVVIKALEDGVNVI GLTRGADTRFHHSEKLDKGE VLIAQFTEHTSAIKVRGKAY IQTRHGVIESEGKK

Data sets:
Data typeCount
13C chemical shifts161
15N chemical shifts57
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRAP subunit A1
2TRAP subunit B1
3TRAP subunit C1
4TRAP subunit D1
5TRAP subunit E1
6TRAP subunit F1
7TRAP subunit G1
8TRAP subunit H1
9TRAP subunit I1
10TRAP subunit J1
11TRAP subunit K1
12L-tryptophan, 12
13L-tryptophan, 22
14L-tryptophan, 32
15L-tryptophan, 42
16L-tryptophan, 52
17L-tryptophan, 62
18L-tryptophan, 72
19L-tryptophan, 82
20L-tryptophan, 92
21L-tryptophan, 102
22L-tryptophan, 112

Entities:

Entity 1, TRAP subunit A 74 residues - 8243 Da.

1   METTYRTHRASNSERASPPHEVALVALILE
2   LYSALALEUGLUASPGLYVALASNVALILE
3   GLYLEUTHRARGGLYALAASPTHRARGPHE
4   HISHISSERGLULYSLEUASPLYSGLYGLU
5   VALLEUILEALAGLNPHETHRGLUHISTHR
6   SERALAILELYSVALARGGLYLYSALATYR
7   ILEGLNTHRARGHISGLYVALILEGLUSER
8   GLUGLYLYSLYS

Entity 2, L-tryptophan, 1 - C11 H12 N2 O2 - 204.225 Da.

1   TRP

Samples:

sample_1: trp RNA-binding attenuation protein, [U-2H; U-13C; U-15N], 6.6 mM; L-TRYPTOPHAN, [U-2H; U-13C; U-15N], 6.6 mM

condition_1: pH: 8.0 na; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
TROSYsample_1not availablecondition_1
TROSY-HNCAsample_1not availablecondition_1
TROSY-HN(CO)CAsample_1not availablecondition_1
TROSY-HNCACBsample_1not availablecondition_1
TROSY-HNCOsample_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAD76498
GenBank AAD33793 ABO67499
REF YP_001126244 YP_148066
SWISS-PROT Q9X6J6