BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5405

Title: STRUCTURE OF SYNTHETIC 26-MER PEPTIDE CONTAINING 145-169 SHEEP PRION PROTEIN SEGMENT AND C-TERMINAL CYSTEINE IN TFE SOLUTION

Authors: Megy, Simon; Bertho, Gildas; Coadou, Gael; Kozin, Sergey; Debey, Pascale; Hui Bon Hoa, Gaston; Girault, Jean-Pierre

Citation: Megy, Simon; Bertho, Gildas; Coadou, Gael; Kozin, Sergey; Debey, Pascale; Hui Bon Hoa, Gaston; Girault, Jean-Pierre. "Structural duality of a peptide fragment from sheep prion protein spanning helix 1 and beta-strand 2: a potential nucleation site for conformational transition to PrPSc"  . ., .-..

Assembly members:
Major Prion Protein, polymer, 26 residues, Formula weight is not available

Natural source:   Common Name: Sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Major Prion Protein: GNDYEDRYYRENMYRYPNQV YYRPVC

Data sets:
Data typeCount
1H chemical shifts201
coupling constants22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1peptide n31

Entities:

Entity 1, peptide n3 26 residues - Formula weight is not available

1   GLYASNASPTYRGLUASPARGTYRTYRARG
2   GLUASNMETTYRARGTYRPROASNGLNVAL
3   TYRTYRARGPROVALCYS

Samples:

sample_1: Major Prion Protein 4.5 mM; sodium phosphate 10 mM; TFE 87%; D2O 13%

sample_cond_1: ionic strength: 15 mM; pH: 6.5 na; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H DQF-COSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H ROESYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
2D 1H-13C HMBCnot availablenot availablenot available

Software:

xwinnmr v2.1 - collection

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 15394 15399 16328 16616 4650 6083 6377 6381 6383 6403 6953 7142
PDB
DBJ BAA07376 BAB69955 BAB69956 BAD51981 BAE91721
EMBL CAA04234 CAA04235 CAA04236 CAA04272 CAA04273
GB AAA68634 AAA68635 AAA68941 AAB03105 AAB50623
PRF 1921132A
REF NP_001009481 NP_001040617 NP_001075490 NP_001124544 NP_001137270
SP O18754 P23907 P40245 P40246 P40247
TPE CAJ43808 CAL59564