BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5407

Title: Solution structure and characterization of the heme chaperone CcmE   PubMed: 12427019

Authors: Arnesano, F.; Banci, L.; Barker, P.; Bertini, I.; Rosato, A.; Su, X.; Viezzoli, M.

Citation: Arnesano, F.; Banci, L.; Barker, P.; Bertini, I.; Rosato, A.; Su, X.; Viezzoli, M.. "Solution Structure and Characterization of the Heme Chaperone CcmE"  Biochemistry 41, 13587-13594 (2002).

Assembly members:
ccme, polymer, 161 residues, Formula weight is not available

Natural source:   Common Name: Shewanella putrefaciens   Taxonomy ID: not available   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Schewanella putrefaciens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ccme: MNPRRKKRLTLAVALIGGVA AIASLLLYALNSNLNLFYTP SEIVNGKTDTGVKPEAGQRI RVGGMVTVGSMVRDPNSLHV QFAVHDSLGGEILVTYDDLL PDLFREGQGIVAQGVLGEDG KLAATEVLAKHDENYMPPEV AEAMGQKHEKLDYSQQKSAT Q

Data sets:
Data typeCount
1H chemical shifts724
13C chemical shifts417
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c maturation protein E1

Entities:

Entity 1, cytochrome c maturation protein E 161 residues - Formula weight is not available

1   METASNPROARGARGLYSLYSARGLEUTHR
2   LEUALAVALALALEUILEGLYGLYVALALA
3   ALAILEALASERLEULEULEUTYRALALEU
4   ASNSERASNLEUASNLEUPHETYRTHRPRO
5   SERGLUILEVALASNGLYLYSTHRASPTHR
6   GLYVALLYSPROGLUALAGLYGLNARGILE
7   ARGVALGLYGLYMETVALTHRVALGLYSER
8   METVALARGASPPROASNSERLEUHISVAL
9   GLNPHEALAVALHISASPSERLEUGLYGLY
10   GLUILELEUVALTHRTYRASPASPLEULEU
11   PROASPLEUPHEARGGLUGLYGLNGLYILE
12   VALALAGLNGLYVALLEUGLYGLUASPGLY
13   LYSLEUALAALATHRGLUVALLEUALALYS
14   HISASPGLUASNTYRMETPROPROGLUVAL
15   ALAGLUALAMETGLYGLNLYSHISGLULYS
16   LEUASPTYRSERGLNGLNLYSSERALATHR
17   GLN

Samples:

Sample_1: ccme, [U-13C; U-15N], 2.0 mM; phosphate 20 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.0 na; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNHBnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available

Software:

XWINNMR v2.6 - processing

xeasy v1.3 - data analysis

DYANA v1.5 - structure solution

CORMA - iterative matrix relaxation

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
GB AAC02697 AAN53344 ABI37308 ABI41227 ABK46471
REF NP_715899 WP_007644472 WP_011070633 WP_011621035 WP_011715470
SP A0KRQ2 Q0HNQ9 Q0I078 Q8EK44