BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5410

Title: Solution structure of novel non-RGD containing short disintegrin by high resolution NMR   PubMed: 12538900

Authors: Moreno-Murciano, Paz; Monleon, Daniel; Calvete, Juan J.; Celda, Bernardo

Citation: Moreno-Murciano, Paz; Monleon, Daniel; Calvete, Juan J.; Celda, Bernardo; Marcinkiewicz, C.. "Amino Acid Sequence and Homology Modeling of Obtustatin, a Novel non-RGD-containing short Disintegrin isolated from the Venom of Vipera lebetina obtusa"  Protein Sci. 12, 366-371 (2003).

Assembly members:
obtustatin, polymer, 41 residues, Formula weight is not available

Natural source:   Common Name: lebetina obtusa   Taxonomy ID: 8703   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Vipera lebetina obtusa

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
obtustatin: CTTGPCCRQCKLKPAGTTCW KTSLTSHYCTGKSCDCPLYP G

Data sets:
Data typeCount
1H chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1obtustatin1

Entities:

Entity 1, obtustatin 41 residues - Formula weight is not available

1   CYSTHRTHRGLYPROCYSCYSARGGLNCYS
2   LYSLEULYSPROALAGLYTHRTHRCYSTRP
3   LYSTHRSERLEUTHRSERHISTYRCYSTHR
4   GLYLYSSERCYSASPCYSPROLEUTYRPRO
5   GLY

Samples:

Sample_1: obtustatin 1.1 mM

Cond_1: pH*: 2.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
mixing time TOCSYs 15,50 and 80 msSample_1not availableCond_1
mixing time NOESYs 50, 100 and 200 msSample_1not availableCond_1

Software:

xwinnmr v3.1 - spectral processing

SPARKY v3.96 - spectral analysis

NMR spectrometers:

  • Bruker AVANCEDMX 500 MHz
  • Bruker AVANCEDMX 600 MHz
  • Bruker AVANCEDMX 800 MHz

Related Database Links:

PDB
SP P83469