BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5464

Title: Solution Structure of a CCHC Zinc Finger from MOZ

Authors: Kwan, A.; Gell, D.; Liew, C.; Mackay, J.

Citation: Kwan, A.; Gell, D.; Liew, C.; Mackay, J.. "Solution Structure of a CCHC Zinc Finger from MOZ"  The BMRB entry is the only known published source for the data..

Assembly members:
Zinc finger protein, polymer, 31 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Zinc finger protein: RLPKLYLCEFCLKYMKSRTI LQQHMKKCGWF

Data sets:
Data typeCount
1H chemical shifts216
coupling constants5

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Monocytic leukemia zinc finger protein1
2Zinc ion2

Entities:

Entity 1, Monocytic leukemia zinc finger protein 31 residues - Formula weight is not available

1   ARGLEUPROLYSLEUTYRLEUCYSGLUPHE
2   CYSLEULYSTYRMETLYSSERARGTHRILE
3   LEUGLNGLNHISMETLYSLYSCYSGLYTRP
4   PHE

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Zinc finger protein 0.5 mM; ZINC (II) ION 0.7 mM; TCEP 1 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 5.6; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - refinement

ARIA v1.1.2 - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAC25728 BAC29621 BAD00088 BAD72833 BAE21185
EMBL CAH89880
GB AAC50662 AAH24786 AAI42660 AAI42960 AAI72379
REF NP_001074618 NP_001092882 NP_001092883 NP_001094040 NP_006757
SP Q5TKR9 Q8BZ21 Q92794
TPG DAA14459