BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5465

Title: NMR solution structure of human Saposin C   PubMed: 14674747

Authors: de Alba, E.; Weiler, S.; Tjandra, N.

Citation: de Alba, E.; Weiler, S.; Tjandra, N.. "Solution structure of human saposin C: pH-dependent interaction with phospholipid vesicles."  Biochemistry 42, 14729-14740 (2003).

Assembly members:
SAPOSIN C, polymer, 84 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SAPOSIN C: SDVYCEVCEFLVKEVTKLID NNKTEKEILDAFDKMCSKLP KSLSEECQEVVDTYGSSILS ILLEEVSPELVCSMLHLCSG LVPR

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts82
1H chemical shifts610

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SAPOSIN C1

Entities:

Entity 1, SAPOSIN C 84 residues - Formula weight is not available

1   SERASPVALTYRCYSGLUVALCYSGLUPHE
2   LEUVALLYSGLUVALTHRLYSLEUILEASP
3   ASNASNLYSTHRGLULYSGLUILELEUASP
4   ALAPHEASPLYSMETCYSSERLYSLEUPRO
5   LYSSERLEUSERGLUGLUCYSGLNGLUVAL
6   VALASPTHRTYRGLYSERSERILELEUSER
7   ILELEULEUGLUGLUVALSERPROGLULEU
8   VALCYSSERMETLEUHISLEUCYSSERGLY
9   LEUVALPROARG

Samples:

sample_1: SAPOSIN C, [U-15N], 1 mM; NaCl10 – 20 mM; H20 90%; D20 10%

sample_2: SAPOSIN C, [U-13C; U-15N], 1 mM; NaCl10 – 20 mM; H20 90%; D20 10%

sample_3: SAPOSIN C, [U-13C; U-15N], 1 mM; NaCl10 – 20 mM; D20 100%

sample_cond_1: ionic strength: 15 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
4D 13C/15N-separated NOESYnot availablenot availablenot available
4D 13C-separated NOESYnot availablenot availablenot available
3D 13C-separated TOCSYnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
experiments for residual dipolar coupling measurementsnot availablenot availablenot available

Software:

xwinnmr v2.5 - collection

NMRPipe v2.1 - processing

PIPP v4.2.8 - data analysis

X-PLOR v3.840 - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 5951 6158
PDB