BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5466

Title: Structural model for an alkaline form of ferricytochrome c   PubMed: 12617658

Authors: Assfalg, M.; Bertini, I.; Dolfi, A.; Turano, P.; Mauk, A.; Rosell, F.; Gray, H.

Citation: Assfalg, M.; Bertini, I.; Dolfi, A.; Turano, P.; Mauk, A.; Rosell, F.; Gray, H.. "Structural Model for an Alkaline form of Ferricytochrome C"  J. Am. Chem. Soc. 125, 2913-2922 (2003).

Assembly members:
Cytochrome c, iso-1, polymer, 95 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cytochrome c, iso-1: LFKTRCLQCHTVEKGGPHKV GPNLHGIFGRHSGQAEGYSY TDANIKKNVLWDENNMSEYL TNPAKYIPGTAMAFGGLKKE KDRNDLITYLKKATE

Data sets:
Data typeCount
15N chemical shifts72
1H chemical shifts414

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cytochrome c, iso-11
2HEME C reduced2

Entities:

Entity 1, Cytochrome c, iso-1 95 residues - Formula weight is not available

1   LEUPHELYSTHRARGCYSLEUGLNCYSHIS
2   THRVALGLULYSGLYGLYPROHISLYSVAL
3   GLYPROASNLEUHISGLYILEPHEGLYARG
4   HISSERGLYGLNALAGLUGLYTYRSERTYR
5   THRASPALAASNILELYSLYSASNVALLEU
6   TRPASPGLUASNASNMETSERGLUTYRLEU
7   THRASNPROALALYSTYRILEPROGLYTHR
8   ALAMETALAPHEGLYGLYLEULYSLYSGLU
9   LYSASPARGASNASPLEUILETHRTYRLEU
10   LYSLYSALATHRGLU

Entity 2, HEME C reduced - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Cytochrome c, iso-1 2 mM; PROTOPORPHYRIN IX CONTAINING FE mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 11.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1

Software:

DYANA v1.5 - structure solution

CORMA - iterative matrix relaxation

AMBER v6.0 - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB