BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5469

Title: Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond

Authors: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji

Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji. "Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond"  Biochemistry 42, 2524-2534 (2003).

Assembly members:
ovomucoid third domain from silver pheasant, polymer, 54 residues, Formula weight is not available

Natural source:   Common Name: silver pheasant   Taxonomy ID: 9046   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lophura nycthemera

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ovomucoid third domain from silver pheasant: AVSVDCSEYPKPACTMEYRP LCGSDNKTYGNKCNFCNAVV ESNGTLTLSHFGKC

Data sets:
Data typeCount
1H chemical shifts343

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1omsvp3 monomer1

Entities:

Entity 1, omsvp3 monomer 54 residues - Formula weight is not available

1   ALAVALSERVALASPCYSSERGLUTYRPRO
2   LYSPROALACYSTHRMETGLUTYRARGPRO
3   LEUCYSGLYSERASPASNLYSTHRTYRGLY
4   ASNLYSCYSASNPHECYSASNALAVALVAL
5   GLUSERASNGLYTHRLEUTHRLEUSERHIS
6   PHEGLYLYSCYS

Samples:

sample_1: ovomucoid third domain from silver pheasant 4.0 mM

Ex-cond_1: pH: 4.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQF-COSYnot availablenot availablenot available

Software:

NMRPipe - processing

NMRPIPP - peak picking, assignment

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 1374 1375 4068 42 4864 507 5411 5424 5425 5426 5427 5428 5429 5448 5518 5520 5521
PDB
PIR A31445 B61588 C31438 E31437 E31442
SP P52245 P52258 P52261 P52269 P67944