BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5480

Title: 1H,15N and 13C assigned Chemical Shifts for a complex of calmodulin with a peptide of the olfactory CNGC channel   PubMed: 12885399

Authors: Orsale, Maria; Melino, Sonia; Torre, Vincent; Motta, Andrea; Paci, Maurizio; Desideri, Alessandro; Cicero, Daniel

Citation: Orsale, Maria; Melino, Sonia; Contessa, G.; Torre, Vincent; Andreotti, G.; Motta, Andrea; Paci, Maurizio; Desideri, Alessandro; Cicero, Daniel. "Two distinct calcium-calmodulin interactions with N-terminal regions of the olfactory and rod cyclic nucleotide-gated channels characterized by NMR spectroscopy"  FEBS Lett. 548, 11-16 (2003).

Assembly members:
Calmodulin, polymer, 148 residues, 16700 Da.
olfactory channel peptide, polymer, 26 residues, 2900 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
olfactory channel peptide: QQRRGGFRRIARLVGVLREW AYRNFR

Data sets:
Data typeCount
1H chemical shifts1141
13C chemical shifts664
15N chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2olfactory channel peptide2
3Calcium ion, I3
4Calcium ion, II3
5Calcium ion, III3
6Calcium ion, IV3

Entities:

Entity 1, calmodulin 148 residues - 16700 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, olfactory channel peptide 26 residues - 2900 Da.

1   GLNGLNARGARGGLYGLYPHEARGARGILE
2   ALAARGLEUVALGLYVALLEUARGGLUTRP
3   ALATYRARGASNPHEARG

Entity 3, Calcium ion, I - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calmodulin, [U-95% 13C; U-95% 15N], 1.2 mM; olfactory channel peptide 1.2 mM; KCl 140 mM; CaCl2 7 mM

sample_2: Calmodulin, [U-15N], 0.4 mM; olfactory channel peptide 0.4 mM; KCl 140 mM; CaCl2 7 mM

Exp-cond_1: pH: 6.7; temperature: 308 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableExp-cond_1
15N edited TOCSY (40ms)not availablenot availableExp-cond_1
15N edited NOESY (80ms)not availablenot availableExp-cond_1
HNHAnot availablenot availableExp-cond_1
1H-15N NOEnot availablenot availableExp-cond_1
HNCAnot availablenot availableExp-cond_1
HN(CO)CAnot availablenot availableExp-cond_1
HNCOnot availablenot availableExp-cond_1
CBCA(CO)NHnot availablenot availableExp-cond_1
HBHA(CO)NHnot availablenot availableExp-cond_1
15N-filtered TOCSY (40 ms)not availablenot availableExp-cond_1
15N-filtered TOCSY (60 ms)not availablenot availableExp-cond_1
15N-13C filtered NOESY (80ms)not availablenot availableExp-cond_1

Software:

NMRPipe v2 -

NMRView v5 -

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
EMBL CAA38754 CAA38754
PRF 1616224A 1616224A
REF NP_001001139 XP_005227654 XP_010841839 NP_001001139
SP Q03041
TPG DAA13266
SWISS-PROT Q03041