BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5481

Title: Backbone and side chain 1H, 13C, and 15N chemical shift assignments for S. cerevisae Hub1

Authors: Ramelot, Theresa; Kennedy, Michael

Citation: Ramelot, Theresa; Cort, R.; Yee, A.; Semesi, A.; Edwards, A.; Arrowsmith, C.; Kennedy, Michael. "Solution structure of the yeast ubiquitin-like modifier protein Hub1"  J. Struct. Funct. Genomics 4, 25-30 (2003).

Assembly members:
Hub1, polymer, 93 residues, 8272 Da.

Natural source:   Common Name: Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Hub1: MGSSHHHHHHSSGLVPRGSH MIEVVVNDRLGKKVRVKCLA EDSVGDFKKVLSLQIGTQPN KIVLQKGGSVLKDHISLEDY EVHDQTNLELYYL

Data sets:
Data typeCount
1H chemical shifts537
13C chemical shifts321
15N chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hub11

Entities:

Entity 1, hub1 93 residues - 8272 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METILEGLUVALVALVALASNASPARGLEU
4   GLYLYSLYSVALARGVALLYSCYSLEUALA
5   GLUASPSERVALGLYASPPHELYSLYSVAL
6   LEUSERLEUGLNILEGLYTHRGLNPROASN
7   LYSILEVALLEUGLNLYSGLYGLYSERVAL
8   LEULYSASPHISILESERLEUGLUASPTYR
9   GLUVALHISASPGLNTHRASNLEUGLULEU
10   TYRTYRLEU

Samples:

sample_1: Hub1, [U-15N; U-13C], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM

sample_2: Hub1, [U-15N], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM

sample_3: Hub1, [U-10% 13C; U-15N], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM

cond_1: pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4D CC-NOESY and 1H-15N HSQC spectra were recorded after exchange into D2O.not availablenot availablecond_1
1H-13C HSQC was recorded on U-15N, 10% 13C labeled samplenot availablenot availablecond_1

Software:

Felix v98 - processing data

XPLOR v3.84 - structure calculations

Sparky v3.98 - chemical shift and noesy assignments

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ GAA26125
EMBL CAY82226
GB AAS56885 AHY77119 EDN62838 EDV12235 EEU08318
PIR S78735
REF NP_014430
SP Q6Q546
TPG DAA10573