BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5482

Title: Assignment of 1H, 13C and 15N resonances of the N-terminal microtubule-binding domain of human doublecortin

Authors: Cierpicki, Tomasz; Kim, Myung Hee; Otlewski, Jacek; Derewenda, Zygmunt; Bushweller, John

Citation: Cierpicki, Tomasz; Kim, Myung Hee; Otlewski, Jacek; Derewenda, Zygmunt; Bushweller, John. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the N-terminal microtubule-binding domain of human doublecortin"  J. Biomol. NMR 25, 81-82 (2003).

Assembly members:
doublecortin, polymer, 113 residues, 12000 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
doublecortin: GAMDPEFALSNEKKAKKVRF YRNGDRYFKGIVYAVSSDRF RSFDALLADLTRSLSDNINL PQGVRYIYTIDGSRKIGSMD ELEEGESYVCSSDNFFKKVE YTKNVNPNWSVNV

Data sets:
Data typeCount
1H chemical shifts793
13C chemical shifts353
15N chemical shifts122
coupling constants94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DC 45-1501

Entities:

Entity 1, DC 45-150 113 residues - 12000 Da.

1   GLYALAMETASPPROGLUPHEALALEUSER
2   ASNGLULYSLYSALALYSLYSVALARGPHE
3   TYRARGASNGLYASPARGTYRPHELYSGLY
4   ILEVALTYRALAVALSERSERASPARGPHE
5   ARGSERPHEASPALALEULEUALAASPLEU
6   THRARGSERLEUSERASPASNILEASNLEU
7   PROGLNGLYVALARGTYRILETYRTHRILE
8   ASPGLYSERARGLYSILEGLYSERMETASP
9   GLULEUGLUGLUGLYGLUSERTYRVALCYS
10   SERSERASPASNPHEPHELYSLYSVALGLU
11   TYRTHRLYSASNVALASNPROASNTRPSER
12   VALASNVAL

Samples:

sample_1: doublecortin, [U-13C; U-15N], 1.0 mM

sample_2: doublecortin, [U-15N], 1.0 mM

DC_cond_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableDC_cond_1
1H-15N TOCSYnot availablenot availableDC_cond_1
HNCOnot availablenot availableDC_cond_1
HNCACBnot availablenot availableDC_cond_1
CBCA(CO)NHnot availablenot availableDC_cond_1
CC(CO)NH-TOCSYnot availablenot availableDC_cond_1
HC(CO)NH-TOCSYnot availablenot availableDC_cond_1
HNHAnot availablenot availableDC_cond_1
HCCH-TOCSYnot availablenot availableDC_cond_1
HACAHBnot availablenot availableDC_cond_1
15N NOESYnot availablenot availableDC_cond_1
13C NOESYnot availablenot availableDC_cond_1

Software:

NMRPipe v2.1 - spectral processing

Sparky v3.100 - assignment and analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAA33387 BAB29230 BAC33328 BAE37637 BAE43338
EMBL CAA05867 CAA06617 CAC38099
GB AAC31696 AAC31797 AAC31799 AAC52037 AAG18479
REF NP_000546 NP_001103692 NP_001103693 NP_001103694 NP_001182482
SP O43602 O88809 Q9ESI7
TPG DAA13014