BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5485

Title: Thermal stability and solution structure of the ribosomal protein L30e from hyperthermophile archaeon Thermococcus celer   PubMed: 12824494

Authors: Chan, Siu-Hong; Leung, Tak-Yuen; Chen, Yu Wai; Bycroft, Mark; Wong, Kam-Bo

Citation: Wong, Kam-Bo; Lee, Chi-Fung; Chan, Siu-Hong; Leung, Tak-Yuen; Chen, Yu Wai; Bycroft, Mark. "Solution Structure and Thermal Stability of Ribosomal Protein L30e from Hyperthermophilic Archaeon Thermococcus celer"  Protein Sci. 12, 1483-1495 (2003).

Assembly members:
Ribosomal protein L30e, polymer, 100 residues, Formula weight is not available

Natural source:   Common Name: T celer   Taxonomy ID: 2264   Superkingdom: Archaea   Kingdom: not available   Genus/species: Thermococcus celer

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Ribosomal protein L30e: VDFAFELRKAQDTGKIVMGA RKSIQYAKMGGAKLIIVARN ARPDIKEDIEYYARLSGIPV YEFEGTSVELGTLLGRPHTV SALAVVDPGESRILALGGKE

Data sets:
Data typeCount
1H chemical shifts700
13C chemical shifts300
15N chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1L30e1

Entities:

Entity 1, L30e 100 residues - Formula weight is not available

1   VALASPPHEALAPHEGLULEUARGLYSALA
2   GLNASPTHRGLYLYSILEVALMETGLYALA
3   ARGLYSSERILEGLNTYRALALYSMETGLY
4   GLYALALYSLEUILEILEVALALAARGASN
5   ALAARGPROASPILELYSGLUASPILEGLU
6   TYRTYRALAARGLEUSERGLYILEPROVAL
7   TYRGLUPHEGLUGLYTHRSERVALGLULEU
8   GLYTHRLEULEUGLYARGPROHISTHRVAL
9   SERALALEUALAVALVALASPPROGLYGLU
10   SERARGILELEUALALEUGLYGLYLYSGLU

Samples:

sample_1: Ribosomal protein L30e 1 mM; D20 100%

sample_2: Ribosomal protein L30e, [U-100% 15N], 1 mM

sample_3: Ribosomal protein L30e, [U-100% 15N; U-100% 13C], 1 mM

sample_4: Ribosomal protein L30e, [U-100% 15N; U-100% 13C], 1 mM; D20 100%

conditions_1: pH: 5.6; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
NOESY-HSQCnot availablenot availablenot available
TOCSY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCAnot availablenot availablenot available
1H-13C HCCH-TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
EMBL CAA42847 CAA47725
SP P29160