BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5494

Title: NMR Structure of PW2 Bound to SDS Micelles: A Tryptophan-rich Anticocidial Peptide Selected from Phage Display Libraries   PubMed: 12130641

Authors: Tinoco, L.; da Silva, A.; Leite, A.; Valente, A.; Almeida, F.

Citation: Tinoco, L.; da Silva, A.; Leite, A.; Valente, A.; Almeida, F.. "NMR Structure of PW2 bound to SDS Micelles. A Tryptophan-rich Anticocidial Peptide selected from Phage Display Libraries"  J. Biol. Chem. 277, 36351-36356 (2002).

Assembly members:
anticocidial peptide, polymer, 12 residues, 1611 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
anticocidial peptide: HPLKQYWWRPSI

Data typeCount
1H chemical shifts266

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cationic peptide1

Entities:

Entity 1, cationic peptide 12 residues - 1611 Da.

1   HISPROLEULYSGLNTYRTRPTRPARGPRO
2   SERILE

Samples:

PW2_1: anticocidial peptide 4.0 mM; sodium_phosphate_buffer 20.0 mM; sodium_chloride 100.0 mM; D2O 10%

PW2-40SDS: anticocidial peptide 4.0 mM; sodium_phosphate_buffer 20.0 mM; SDS 40.0 mM; sodium_chloride 100.0 mM

PW2-160SDS: anticocidial peptide 4.0 mM; sodium_phosphate_buffer 20.0 mM; sodium_chloride 100.0 mM; SDS 160.0 mM; D2O 10.0%

sample_conds: pH: 5.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_conds
2D TOCSYnot availablenot availablesample_conds

Software:

NMRPipe - processing

NMRVIEW v4.1.2 - data analysis

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker DRX 400 MHz

Related Database Links:

BMRB 5494
PDB