BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5499

Title: The PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area   PubMed: 12196542

Authors: Feng, Wei; Fan, Jing-Song; Jiang, Ming; Shi, Ya-Wei; Zhang, Mingjie

Citation: Feng, Wei; Fan, Jing-Song; Jiang, Ming; Shi, Ya-Wei; Zhang, Mingjie. "PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area"  J. Biol. Chem. 277, 41140-41146 (2002).

Assembly members:
the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
the seventh PDZ domain of Glutamate Receptor Interacting Protein 1: SPTPVELHKVTLYKDSGMED FGFSVADGLLEKGVYVKNIR PAGPGDLGGLKPYDRLLQVN HVRTRDFDCCLVVPLIAESG NKLDLVISRNP

Data sets:
Data typeCount
1H chemical shifts584
13C chemical shifts369
15N chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AMPA receptor interacting protein1

Entities:

Entity 1, AMPA receptor interacting protein 91 residues - Formula weight is not available

1   SERPROTHRPROVALGLULEUHISLYSVAL
2   THRLEUTYRLYSASPSERGLYMETGLUASP
3   PHEGLYPHESERVALALAASPGLYLEULEU
4   GLULYSGLYVALTYRVALLYSASNILEARG
5   PROALAGLYPROGLYASPLEUGLYGLYLEU
6   LYSPROTYRASPARGLEULEUGLNVALASN
7   HISVALARGTHRARGASPPHEASPCYSCYS
8   LEUVALVALPROLEUILEALAGLUSERGLY
9   ASNLYSLEUASPLEUVALILESERARGASN
10   PRO

Samples:

sample_1: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1 1.0 mM; D2O 99.9%; potassium phosphate buffer 100 mM

sample_2: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, [U-100% 15N], 1 mM; H2O 90%; D20 10%; potassium phosphate buffer 100 mM

sample_3: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, [U-100% 15N;U-100% 13C], 1.0 mM; D2O 99.9%; potassium phosphate buffer 100 mM

sample_4: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, [U-100% 15N; U-100% 13C], 1.0 mM; H2O 90%; D2O 10%; potassium phosphate buffer 100 mM

sample_cond_1: pH: 6.5 na; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 15N-separated_NOESYnot availablenot availablenot available
3D 13C-separated_NOESYnot availablenot availablenot available

Software:

CNS v1.1 - structure solution

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAC26668
EMBL CAB39895
GB AAP81257 AAQ56581 AAR08916 ABB46288 EDL24394
REF NP_001264224 XP_002687693 XP_004007495 XP_004330097 XP_005962414
TPG DAA29720