BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5503

Title: 1H resonance assignment of the C-terminal domain of Human Centrin2 (LC-HsCen2: M84-Y172)   PubMed: 12578356

Authors: Matei, E.; Miron, S.; Blouquit, Y.; Duchambon, P.; Durussel, P.; Cox, J.; Craescu, C.

Citation: Matei, E.; Miron, S.; Blouquit, Y.; Duchambon, P.; Durussel, P.; Cox, J.; Craescu, C.. "C-terminal Half of Human Centrin 2 Behaves like a Regulatory EF-hand Domain"  Biochemistry 42, 1439-1450 (2003).

Assembly members:
Human centrin2, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Human centrin2: MNFGDFLTVMTQKMSEKDTK EEILKAFKLFDDDETGKISF KNLKRVAKELGENLTDEELQ EMIDEADRDGDGEVSEQEFL RIMKKTSLY

Data sets:
Data typeCount
1H chemical shifts540

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human centrin21

Entities:

Entity 1, Human centrin2 89 residues - Formula weight is not available

1   METASNPHEGLYASPPHELEUTHRVALMET
2   THRGLNLYSMETSERGLULYSASPTHRLYS
3   GLUGLUILELEULYSALAPHELYSLEUPHE
4   ASPASPASPGLUTHRGLYLYSILESERPHE
5   LYSASNLEULYSARGVALALALYSGLULEU
6   GLYGLUASNLEUTHRASPGLUGLULEUGLN
7   GLUMETILEASPGLUALAASPARGASPGLY
8   ASPGLYGLUVALSERGLUGLNGLUPHELEU
9   ARGILEMETLYSLYSTHRSERLEUTYR

Samples:

sample_one: Human centrin2 1.5 mM; Tris_d11, [U-2H], 20 mM; NaCl 100 mM; H2O 95%; D2O 5%

sample_two: Human centrin2 1.4 mM; Tris_d11, [U-2H], 20 mM; NaCl 100 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 6.5; temperature: 308 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

DGII vI2000 - structure solution

DISCOVER vI2000 - refinement

NMR spectrometers:

  • Varian UNITY 500 MHz

Related Database Links:

BMRB 15755
PDB
GB EPY78459 ERE65656