BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5511

Title: Backbone 1H, 13C, and 15N resonance assignments for the 21 kDa GTPase Rac1 complexed to GDP and Mg2+

Authors: Thapar, Roopa; Moore, Cathy; Campbell, Sharon

Citation: Thapar, Roopa; Moore, Cathy; Campbell, Sharon. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments for the 21 kDa GTPase Rac1 complexed to GDP and Mg2+"  J. Biomol. NMR 27, 87-88 (2003).

Assembly members:
Rac1, polymer, 188 residues, 20991.4 Da.
GDP, non-polymer, 443.201 Da.
MG, non-polymer, 24.305 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Rac1: MQAIKCVVVGDGAVGKTCLL ISYTTNAFPGEYIPTVFDNY SANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLI CFSLVSPASFENVRAKWYPE VRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLTPITYP QGLAMAKEIGAVKYLECSAL TQRGLKTVFDEAIRAVLSPP PVKKRKRK

Data sets:
Data typeCount
1H chemical shifts138
13C chemical shifts261
15N chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rac11
2Guanosine-5'-diphosphate2
3Magnesium ion3

Entities:

Entity 1, Rac1 188 residues - 20991.4 Da.

1   METGLNALAILELYSCYSVALVALVALGLY
2   ASPGLYALAVALGLYLYSTHRCYSLEULEU
3   ILESERTYRTHRTHRASNALAPHEPROGLY
4   GLUTYRILEPROTHRVALPHEASPASNTYR
5   SERALAASNVALMETVALASPGLYLYSPRO
6   VALASNLEUGLYLEUTRPASPTHRALAGLY
7   GLNGLUASPTYRASPARGLEUARGPROLEU
8   SERTYRPROGLNTHRASPVALPHELEUILE
9   CYSPHESERLEUVALSERPROALASERPHE
10   GLUASNVALARGALALYSTRPTYRPROGLU
11   VALARGHISHISCYSPROASNTHRPROILE
12   ILELEUVALGLYTHRLYSLEUASPLEUARG
13   ASPASPLYSASPTHRILEGLULYSLEULYS
14   GLULYSLYSLEUTHRPROILETHRTYRPRO
15   GLNGLYLEUALAMETALALYSGLUILEGLY
16   ALAVALLYSTYRLEUGLUCYSSERALALEU
17   THRGLNARGGLYLEULYSTHRVALPHEASP
18   GLUALAILEARGALAVALLEUSERPROPRO
19   PROVALLYSLYSARGLYSARGLYS

Entity 2, Guanosine-5'-diphosphate - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, Magnesium ion - Mg - 24.305 Da.

1   MG

Samples:

Rac1_GDP_Mg_(15N_13C_2H): Rac1, [U-15N; U-13C; U-2H], 0.5 – 0.7 mM

condition_1: pH: 6.8; temperature: 298 K; ionic strength: 0.065 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HNCARac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HN(CO)CARac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HNCORac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
1H-15N TOCSY-HSQCRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
CBCA(CO)NHRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HNCACBRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
CC(CO)NH TOCSYRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
1H-15N NOESY-HSQCRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1

Software:

FELIX v98.2 - data processing, analysis

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

SWISS-PROT Q6RUV5 P63001 P63000 P62999 P62998
REF NP_001089332 NP_001084224 NP_001034907 NP_001004840 NP_001003274
GenBank AAD50299 AAC18960 AAB22206 AAA36544 AAA36537
EMBL CAJ83626 CAG04437 CAB53579 CAA40545 CAA39801
DBJ BAC28767 BAC16311 BAB69451 BAB26027 BAB25667
PDB
BMRB 6970 11010