BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5517

Title: NMR studies of the DNA-binding domain of B-Myb   PubMed: 12815265

Authors: Jones, Gareth

Citation: Jones, Gareth; Howard, Mark; McIntosh, Pauline; Williamson, Richard; Carr, Mark. "Sequence-specific Assignment of the B-Myb DNA-binding Domain (B-MybR2R3) bound to a 16 Base-pair DNA Target Site Corresponding to a Regulatory Site from the tom-1 Gene "  J. Biomol. NMR 26, 375-376 (2003).

Assembly members:
B-MybR2R3-tom1a, polymer, 110 residues, 13095 Da.
tom1a, polymer, 16 residues, 4879 Da.
tom1a, polymer, 16 residues, 4879 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
B-MybR2R3-tom1a: GIPDLVKGPWTKEEDQKVIE LVKKYGTKQWTLIAKHLKGR LGKQCRERWHNHLNPEVKKS SWTEEEDRIIFEAHKVLGNR WAEIAKLLPGRTDNAVKNHW NSTIKRKVDT
tom1a: CTCAGTCCGTTAAGGA
tom1a: TCCTTAACGGACTGAG

Data sets:
Data typeCount
1H chemical shifts625
13C chemical shifts289
15N chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B-MybR2R3 unit 11
2tom1a 16mer2
3tom1a 16mer3

Entities:

Entity 1, B-MybR2R3 unit 1 110 residues - 13095 Da.

1   GLYILEPROASPLEUVALLYSGLYPROTRP
2   THRLYSGLUGLUASPGLNLYSVALILEGLU
3   LEUVALLYSLYSTYRGLYTHRLYSGLNTRP
4   THRLEUILEALALYSHISLEULYSGLYARG
5   LEUGLYLYSGLNCYSARGGLUARGTRPHIS
6   ASNHISLEUASNPROGLUVALLYSLYSSER
7   SERTRPTHRGLUGLUGLUASPARGILEILE
8   PHEGLUALAHISLYSVALLEUGLYASNARG
9   TRPALAGLUILEALALYSLEULEUPROGLY
10   ARGTHRASPASNALAVALLYSASNHISTRP
11   ASNSERTHRILELYSARGLYSVALASPTHR

Entity 2, tom1a 16mer 16 residues - 4879 Da.

1   DCDTDCDADGDTDCDCDGDT
2   DTDADADGDGDA

Entity 3, tom1a 16mer 16 residues - 4879 Da.

1   DTDCDCDTDTDADADCDGDG
2   DADCDTDGDADG

Samples:

sample_1: B-MybR2R3-tom1a, [U-15N; U-13C]-All but Pro, 1.9 mM; tom1a 1.9 mM; tom1a 1.9 mM

Ex-cond_1: pH: 6.2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
1H-15N HSQCsample_1not availableEx-cond_1

Software:

XEasy - peak picking, manual assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4114
PDB
DBJ BAC25979 BAG37322 BAH13925 BAI46683
EMBL CAA31655 CAA47839 CAA49898
GB AAH07585 AAH50842 AAH53555 AAI18124 AAP36828
REF NP_001068916 NP_001100006 NP_001231398 NP_001252784 NP_001265539
SP P10244 P48972 Q03237
TPG DAA23198