BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5527

Title: Three-Dimensional Solution Structure of Huwentoxin-Iv by 2D 1H-NMR   PubMed: 12228241

Authors: Peng, K.; Shu, Q.; Liang, S.

Citation: Peng, K.; Shu, Q.; Liu, Z.; Liang, S.. "Function and Solution Structure of Huwentoxin-IV, a Potent Nueronal Tetrodotoxin (TTX)-sensitive Sodium Channel Antagonist from Chinese Bird Spider Selenocosmia huwena"  J. Biol. Chem. 277, 47564-47571 (2002).

Assembly members:
Huwentoxin-IV, polymer, 35 residues, Formula weight is not available

Natural source:   Common Name: Chinese bird spider   Taxonomy ID: 29017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ornithoctonus huwena

Experimental source:   Production method: .

Entity Sequences (FASTA):
Huwentoxin-IV: ECLEIFKACNPSNDQCCKSS KLVCSRKTRWCKYQI

Data sets:
Data typeCount
1H chemical shifts250

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1huwentoxin-iv1

Entities:

Entity 1, huwentoxin-iv 35 residues - Formula weight is not available

1   GLUCYSLEUGLUILEPHELYSALACYSASN
2   PROSERASNASPGLNCYSCYSLYSSERSER
3   LYSLEUVALCYSSERARGLYSTHRARGTRP
4   CYSLYSTYRGLNILE

Samples:

sample_1: Huwentoxin-IV 5 mM; phosphate buffer mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.50; temperature: 300.00 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
TOCSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

X-PLOR v3.851 - refinement

FELIX v98.0 - structure solution

NMR spectrometers:

  • BRUKER DMX 500 MHz

Related Database Links:

BMRB 19026 19030 19032
PDB
GB AAP33074 ABY77744 ABY77745 ABY77746
SP P83303