BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5529

Title: The structure of G16A MNEI monellin supports a new model of interaction of sweet proteins with the T1R2-T1R3 receptor   PubMed: 12706725

Authors: Spadaccini, R.; Trabucco, F.; Saviano, G.; Picone, D.; Crescenzi, O.; Tancredi, T.; Temussi, P.

Citation: Spadaccini, R.; Trabucco, F.; Saviano, G.; Picone, D.; Crescenzi, O.; Tancredi, T.; Temussi, P.. "The Mechanism of Interaction of Sweet Proteins with the T1R2-T1R3 Receptor: Evidence from the Solution Structure of G16A-MNEI"  J. Mol. Biol. 328, 683-692 (2003).

Assembly members:
monellin, polymer, 97 residues, Formula weight is not available

Natural source:   Common Name: serendipity berry   Taxonomy ID: 3457   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Dioscoreophyllum cumminsii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
monellin: MGEWEIIDIGPFTQNLAKFA VDEENKIGQYGRLTFNKVIR PCMKKTIYENEGFREIKGYE YQLYVYASDKLFRADISEDY KTRGRKLLRFNGPVPPP

Data sets:
Data typeCount
15N chemical shifts104
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Monellin chains B and A1

Entities:

Entity 1, Monellin chains B and A 97 residues - Formula weight is not available

1   METGLYGLUTRPGLUILEILEASPILEGLY
2   PROPHETHRGLNASNLEUALALYSPHEALA
3   VALASPGLUGLUASNLYSILEGLYGLNTYR
4   GLYARGLEUTHRPHEASNLYSVALILEARG
5   PROCYSMETLYSLYSTHRILETYRGLUASN
6   GLUGLYPHEARGGLUILELYSGLYTYRGLU
7   TYRGLNLEUTYRVALTYRALASERASPLYS
8   LEUPHEARGALAASPILESERGLUASPTYR
9   LYSTHRARGGLYARGLYSLEULEUARGPHE
10   ASNGLYPROVALPROPROPRO

Samples:

sample_1: monellin 2 mM; pottasium phosphate buffer 18.5 mM; H2O 90%; D2O 10%

sample_2: monellin, [U-15N], 2 mM; pottasium phosphate buffer 18.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 18.5 mM; pH: 2.9; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
3D 15N-separated TOCSYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HNHBnot availablenot availablesample_cond_1
HSQCnot availablenot availablesample_cond_1

Software:

NMRPipe v1.7 - processing

NMRView v4.0.3 - data analysis

DYANA v1.5 - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 4638
PDB
GB AFF58925
PRF 761801B