BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5530

Title: Naturally occurring modifications reduces the anticodon domain conformational space of tRNA-PHE   PubMed: 14643656

Authors: Stuart, J.; Koshlap, K.; Guenther, R.; Agris, P.

Citation: Stuart, J.; Koshlap, K.; Guenther, R.; Agris, P.. "Naturally occurring modifications restricts the anticodon domain conformational space of tRNA-PHE"  J. Mol. Biol. 334, 901-918 (2003).

Assembly members:
5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3', polymer, 17 residues, Formula weight is not available
OMC, non-polymer, 337.223 Da.
OMG, non-polymer, 377.247 Da.
5MC, non-polymer, 337.223 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3': CCAGAXUXAAGAUXUGG

Data sets:
Data typeCount
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3'1

Entities:

Entity 1, 5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3' 17 residues - Formula weight is not available

1   CCAGAOMCUOMGAA
2   GAU5MCUGG

Samples:

sample_1: 5'-R(*CP*CP*AP*GP*AP*(OMC)P*UP*(OMG)P*AP*AP*GP*AP*UP*(5MC)P*UP*GP*G)-3' 1.6 mM; cacodylate buffer 10 mM; EDTA 0.1 mM

sample_cond_1: pH: 5.60; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1

Software:

xwinnmr - collection

FELIX v98 - data analysis

DISCOVER v98 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links: